陈坤, 张晶, 洪亮, 贾敏, 陶静, 梁敏红. 上海市虹口区HIV-1感染者分子流行病学研究[J]. 上海预防医学, 2022, 34(9): 854-859. DOI: 10.19428/j.cnki.sjpm.2022.21920
引用本文: 陈坤, 张晶, 洪亮, 贾敏, 陶静, 梁敏红. 上海市虹口区HIV-1感染者分子流行病学研究[J]. 上海预防医学, 2022, 34(9): 854-859. DOI: 10.19428/j.cnki.sjpm.2022.21920
CHEN Kun, ZHANG Jing, HONG Liang, JIA Min, TAO Jing, LIANG Minhong. Molecular epidemiological study on HIV-1 patients in Hongkou District, Shanghai[J]. Shanghai Journal of Preventive Medicine, 2022, 34(9): 854-859. DOI: 10.19428/j.cnki.sjpm.2022.21920
Citation: CHEN Kun, ZHANG Jing, HONG Liang, JIA Min, TAO Jing, LIANG Minhong. Molecular epidemiological study on HIV-1 patients in Hongkou District, Shanghai[J]. Shanghai Journal of Preventive Medicine, 2022, 34(9): 854-859. DOI: 10.19428/j.cnki.sjpm.2022.21920

上海市虹口区HIV-1感染者分子流行病学研究

Molecular epidemiological study on HIV-1 patients in Hongkou District, Shanghai

  • 摘要:
    目的 分析上海市虹口区新确认未治疗人类免疫缺陷病毒(HIV)⁃1感染者的HIV⁃1亚型分布特征,为虹口区建立HIV⁃1亚型数据库,探索获得性免疫缺陷综合征(AIDS)精准防治策略提供基础数据和思路。
    方法 虹口区2016年新确认的HIV⁃1感染者的血浆样品119份,对HIV⁃1 RNA pol区基因进行反转录和基因扩增,扩增后进行DNA测序,在Los Alamos2022 HIV⁃1基因数据库网站上使用HIV⁃BLAST工具进行HIV⁃1亚型分析,并结合流行病学资料进行分析。
    结果 119份样品中,共获得89例pol区基因片段,以CRF07_BC(38.2%)、CRF01_AE(36.0%)为主,B亚型占13.5%,并发现新型重组亚型CRF55_01B和独特重组亚型URF_0107各2例(2.3%)。
    结论 2016年,虹口区HIV⁃1感染者HIV⁃1流行亚型以CRF07_BC和CRF01_AE为主,新的重组亚型CRF55_01B和独特重组亚型URF_0107已在虹口区出现,密切监视其流行趋势十分必要。

     

    Abstract:
    Objective To determine the characteristics of human immunodeficiency virus type 1 (HIV-1) subtypes among newly confirmed HIV-1 patients in Hongkou District of Shanghai, and provide evidence for establishing HIV-1 subtype database and precise prevention and control strategy against AIDS in Hongkou District of Shanghai.
    Methods A total of 119 plasma samples from newly confirmed HIV-1 patients in Hongkou District in 2016 were used to detect HIV-1 RNA pol gene by using reverse transcription PCR. HIV-1 subtypes were determined with Los Alamos 2022 HIV-1 gene database using HIV-BLAST tool, and then characterized by epidemiological data.
    Results In the 119 samples, a total of 89 pol gene fragments were obtained, which were mainly classified as CRF07_BC (38.2%), CRF01_AE (36.0%), and subtype B (13.5%). Furthermore, two new recombinant subtype CRF55_01B and two unique recombinant subtype URF_0107 were identified (2.3%).
    Conclusion In 2016, HIV-1 subtypes in Hongkou District are mainly CRF07_BC and CRF01_AE. In addition, new recombinant subtype CRF55_01B and unique recombinant subtype URF_0107 have appeared in Hongkou District. It warrants a need of enhanced surveillance.

     

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