高脂小鼠脂肪肝组织油红O特殊染色的应用

钟晓怡; 林云; 郭艳; 蒲蕊; 曹广文; 殷建华

doi:10.19428/j.cnki.sjpm.2018.18722

高脂小鼠脂肪肝组织油红O特殊染色的应用

Oil-red O special staining techniques applied in fatty liver tissue of hyperlipidemic mouse

摘要

摘要:
目的探讨2种油红O染色法在非酒精性脂肪肝病中脂肪变性检测的应用。
方法选取非酒精性脂肪肝病动物疾病模型C57BL/6J小鼠30只，安乐死处理后摘取左肝叶，将肝组织用4%多聚甲醛固定液充分固定后分为2份进行组织学制片，1份样品制作石蜡切片，石蜡切片制备完成后进行苏木精-伊红(HE)染色，作为脂肪变性的鉴定，另1份样品用30%蔗糖溶液脱水后用OCT包埋制作冰冻切片，冰冻切片制备完成后进行2种方法的油红O染色。通过盲测的方法，在光学显微镜下观察油红O在脂滴细胞的定位情况，并对2种染色方法进行对比。
结果2种染色方法对组织及细胞没有任何病理性的损伤，并具有较好的显色与定位作用。
结论运用良好的特殊染色技术，可以清晰分辨脂滴细胞，并显示出脂滴细胞的数量，从而对非酒精性脂肪肝病中的脂肪变性进行定位与半定量的分析。

Abstract:
ObjectiveTo explore the application of two oil-red staining methods for steatosis in non-alcoholic fatty liver disease.
MethodsThirty C57BL/6J mice models of non-alcoholic fatty disease were selected. After euthanasia, the left liver lobe was harvested immediately, which was immersed in a large volume of 4% paraformaldehyde fixative in cacodylate buffer. After fixation, the liver was divided into two samples for making histological sections. One sample was made into paraffin sections for hematoxyin-eosin (HE) staining to identify steatosis. The other was made into frozen sections of OCT-embedded block after dehydration with 30% sucrose solution. The frozen sections were stained by two oil-red O staining methods. The localization of oil-red O in the lipid droplet was observed by blind measurement under microscope, and the results obtained by the two staining methods were compared.
ResultsThe two staining methods proved to be without pathological damage to tissue and cell, and had good coloration and localization effect, which provided scientific basis for the diagnosis of non-alcoholic fatty liver disease.
ConclusionBy the use of good special staining technology, the lipid drop cells are distinguished clearly, and the number of lipid drop cells are displayed. The steatosis in non-alcoholic fatty disease is located and analyzed with half quantification.

HTML全文

参考文献(19)

施引文献

资源附件(0)