浙江省某地海产品中副溶血性弧菌污染及耐药性分析

毛争争; 陈欢; 郑琼; 贺力; 陆欣

doi:10.19428/j.cnki.sjpm.2021.20168

浙江省某地海产品中副溶血性弧菌污染及耐药性分析

Contamination and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood in a place of Zhejiang Province

摘要

摘要:
目的了解浙江省某区市售海产品中副溶血性弧菌污染状况及耐药性，为防控副溶血性弧菌引起的食源性疾病提供基础数据。
方法根据GB 4789.7—2013标准对海产品中副溶血性弧菌进行分离鉴定，利用实时荧光聚合酶链反应（PCR）法鉴定毒力基因，采用K-B法进行药敏试验。
结果从210份样品中检出副溶血性弧菌阳性样本55份，检出率为26.19%，不同海产品种类检出率差异较大，腹足类和双壳类污染较为严重。分离所得的55株副溶血性弧菌均不含毒力基因tdh和trh，所有菌株均检出2种特异性基因tlh和toxR。大部分菌株对氨苄西林和阿莫西林耐药，耐药率均为96.36％。所有菌株对氨苄西林/舒巴坦、四环素、环丙沙星、左氧氟沙星、氧氟沙星、氯霉素完全敏感。
结论市售海产品普遍存在副溶血性弧菌污染，分离菌株存在一定程度的耐药情况，应加强海产品副溶血性弧菌污染状况监测，规范养殖和临床抗生素使用。

Abstract:
ObjectiveTo understand the contamination and antimicrobial resistance of Vibrio parahaemolyticus in seafood sold in a place of Zhejiang Province， and to provide basic data for the prevention and control of food-borne diseases caused by V. parahaemolyticus.
MethodsV. parahaemolyticus was isolated and identified from seafood according to GB 4789.7—2013 method. The virulence genes were identified by real-time fluorescent PCR， and the antimicrobial sensitivity test was performed by Kirby-Bauer method.
ResultsV. parahaemolyticus was isolated from 55 of 210 seafood samples. The detection rate was 26.19%， and the detection rates of different species were quite different. None of the 55 isolates contained virulence genes tdh and trh， while all isolates have species-specific genes tlh and toxR. Most isolates were resistant to ampicillin and amoxicillin， with the same antimicrobial resistance rate of 96.36%. All isolates were fully sensitive to ampicillin/sulbactam， tetracycline， ciprofloxacin， levofloxacin， loxacin and chloramphenicol.
ConclusionV. parahaemolyticus contamination is common in sold seafood. The antimicrobial resistance of the isolates has reached a certain level. Monitoring of the pollution status of V. parahaemolyticus in seafood should be strengthened and the aquaculture and clinical use of antibiotics should be standardized.

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