赵宏伟, 谢晓红, 陶力新, 沈春莉, 王洁, 沈莉. 上海市奉贤区210株副溶血性弧菌毒力基因、分子分型及耐药性研究[J]. 上海预防医学, 2022, 34(9): 901-905. DOI: 10.19428/j.cnki.sjpm.2022.21765
引用本文: 赵宏伟, 谢晓红, 陶力新, 沈春莉, 王洁, 沈莉. 上海市奉贤区210株副溶血性弧菌毒力基因、分子分型及耐药性研究[J]. 上海预防医学, 2022, 34(9): 901-905. DOI: 10.19428/j.cnki.sjpm.2022.21765
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ZHAO Hongwei, XIE Xiaohong, TAO Lixin, SHEN Chunli, WANG Jie, SHEN Li. Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai[J]. Shanghai Journal of Preventive Medicine, 2022, 34(9): 901-905. DOI: 10.19428/j.cnki.sjpm.2022.21765
Citation: ZHAO Hongwei, XIE Xiaohong, TAO Lixin, SHEN Chunli, WANG Jie, SHEN Li. Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai[J]. Shanghai Journal of Preventive Medicine, 2022, 34(9): 901-905. DOI: 10.19428/j.cnki.sjpm.2022.21765
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上海市奉贤区210株副溶血性弧菌毒力基因、分子分型及耐药性研究

Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai

    摘要
  • 摘要:
    目的 分析上海市奉贤区不同来源副溶血性弧菌耐药性、主要毒力基因携带及分子分型情况。
    方法 对2018—2020年上海市奉贤区分离到的210株副溶血性弧菌进行17种抗生素药物敏感试验，耐热直接溶血毒素基因（thermostable direct hemolysin，tdh）、耐热相关溶血毒素基因（thermostable direct hemolysin⁃related hemolysin，trh）和不耐热溶血素基因（thermolabile hemolysin，tlh）检测以及脉冲场凝胶电泳分型分析。
    结果 药敏结果显示，共检出耐药株147株，耐药率为70.00%。其中，126株对1种抗生素耐药，以耐头孢唑啉（CFZ）为主；15株对2种抗生素耐药，以耐CFZ和氨苄西林（AMP）为主；多重耐药菌（MDR）6株，以耐AMP、复方新诺明（SXT）、CFZ、头孢噻肟（CTX）、头孢他啶（CAZ）和头孢吡肟（FEP）为主。食源性菌株AMP耐药水平高于人源性菌株（P<0.05）。210株副溶血性弧菌均携带毒力基因tlh。84株人源副溶血性弧菌菌株tdh和trh基因携带率分别为89.29%和7.14%；126株食品源副溶血性弧菌菌株trh基因携带率为1.59%，未检出tdh基因。脉冲场凝胶电泳（PFGE）分析得到173种带型，菌株之间的相似度为48.80%~100.00%，存在主要流行克隆系。
    结论 上海市奉贤区副溶血性弧菌存在多重耐药现象，对β⁃内酰胺类抗生素耐药情况较为严重，人源性菌株毒力基因携带率较高。PFGE带型总体上较为分散，但人源菌株中存在主要流行克隆系。奉贤区引起食源性疾病的风险性较高，应加强防控。

     

    Abstract:
    Objective To determine the drug resistance， main virulence genes and molecular typing of Vibrio parahaemolyticus from multiple sources in Fengxian District， Shanghai.
    Methods A total of 210 strains of Vibrio parahaemolyticus were isolated in Fengxian District， Shanghai， from 2018 to 2020. Then drug susceptibility tests of 17 antibiotics， virulence gene detection of thermolabilehemolysin （tlh）， thermostable direct hemolysin （tdh） and thermostable direct hemolysin-relatedhemolysin （trh）， and pulsed-field gel electrophoresis （PFGE） were conducted.
    Results A total of 147 drug-resistant strains were detected， with the drug resistance of 70.00%. Among them， 126 strains were resistant to single antibiotic， mainly resistant to CFZ； 15 strains were resistant to two antibiotics， mainly resistant to CFZ and AMP； and 6 strains of MDR， mainly resistant to AMP， SXT， CFZ， CTX， CAZ and FEP. The AMP resistance of food-borne strains was higher than that of human-derived strains （P<0.05）. All 210 strains of Vibrio parahaemolyticus carried the virulence gene tlh. Furthermore， 84 human-derived strains carried tdh （89.29%） and trh （7.14%）. In contrast， 126 food-borne strains carried trh （1.59%）， while did not carry tdh. In additionally， 173 band patterns were determined by PFGE， sharing the similarity of 48.80%‒100.00%， among which major clones were prevalent.
    Conclusion Vibrio parahaemolyticus in Fengxian District has multi-drug resistance， especially the resistance to β-lactam antibiotics. The prevalence of virulence gene remains high in human-derived strains. Furthermore， major clones are prevalent in human-derived strains， though the PFGE banding patterns are generally diverse. Due to high risk of food-borne diseases， this study warrants strengthening the prevention and control against Vibrio parahaemolyticus.

     

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