Objective To investigate the prevalence of hepatitis C virus （HCV） and genotypes in blood donors in Huzhou， and provide evidence for establishing early warning mechanism and reducing the risk of HCV transmission through blood transfusion.

Methods A total of 108 011 blood samples from donors were tested for anti-HCV antibody and HCV RNA from 2017 to 2021 in Huzhou. Furthermore， HCV genotypes were determined in the positive samples. Statistical analysis was then conducted.

Results Among 108 011 blood donors， 33 were positive for HCV， with an overall prevalence of 0.031%. Univariate analysis showed that gender， history of blood donation， occupation， and marital status were significantly associated with HCV prevalence in voluntary blood donors. Further multivariate logistic regression showed that history of blood donation， gender， and marital status were included in the final model， with OR values of 46.075， 6.261， and 5.204， respectively. A total of four genotypes and five subtypes were identified， among which subtype 1b was the highest （39.34%）， followed by 2a （24.59%）.

Conclusion HCV infection in blood donors is low in Huzhou. However， it remains crucial to strengthen the pre-blood donation intervention for those who are male， aged 25‒34 and 45 and above， and with no regular employment to improve the blood donation quality. In addition， multiple HCV genotypes and subtypes are prevalent in Huzhou， with subtype 1b being the most common type.