三种金黄色葡萄球菌选择性分离培养基的检测效果比较

顾其芳; 张红芝; 朱颖莹; 鲁珺琰; 於颖; 刘诚; 刘玥; 陈敏

doi:10.19428/j.cnki.sjpm.2018.18378

三种金黄色葡萄球菌选择性分离培养基的检测效果比较

Detection effects compared between three types of selective media for Staphylococcus aureus

摘要

摘要:
目的比较三种金黄色葡萄球菌选择性分离培养基的检测效果。
方法分别使用Baird-Parker培养基(BP)、兔血浆纤维蛋白原培养基(RPF)和科玛嘉葡萄球菌显色培养基(CHROMagar^TMStaph aureus)对标准菌株、食品分离株以及食品样品进行对比检测。
结果三种选择性培养基对金黄色葡萄球菌的回收率均达到95%以上；RPF和CHROMagar^TMStaph aureus培养基的检测效率明显高于BP培养基；且两种培养基敏感性、特异性均优于传统的BP培养基。
结论应用RPF和CHROMagar^TMStaph aureus培养基检测食品中金黄色葡萄球菌，较传统的BP培养基具有更好的检测效果，建议将RPF和CHROMagar^TMStaph aureus培养基作为可靠的分离检测培养基应用于食品中金黄色葡萄球菌的分离检测。

Abstract:
ObjectiveTo compare the detection effects between three kinds of selective media for Staphylococcus aureus.
MethodsBaird-Parker (BP) agar, Rabbit Plasma Fibrinogen (RPF) agar and CHROMagar^TMStaph aureus were selected to test the standard strains, food-isolated strains as well as food samples.
ResultsMore than 95% recovery efficiency could be reached by utilization of all the three types of media. However, the detection effects of RPF and CHROMagar^TMStaph aureus were significantly higher than that of BP agar. Moreover, their sensitivity and specificity were also superior to that of conditional BP agar.
ConclusionBetter detection effect is obtained from RPF and CHROMagar^TMStaph aureus when compared to that from BP agar, suggesting that RPF and CHROMagar^TMStaph aureus agar are more reliable for the detection of Staphylococcus aureus in food.

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