腺病毒<b>55</b>型感染致急性结膜炎病例的病原学鉴定及全基因序列分析

刘嘉婧; 崔晓青; 张万菊; 房方皓; 彭雅君; 陈敏; 张靖翊; 滕峥

doi:10.19428/j.cnki.sjpm.2023.22650

腺病毒55型感染致急性结膜炎病例的病原学鉴定及全基因序列分析

Identification and complete genome sequencing of human adenovirus type 55 isolated from a patient with acute hemorrhagic conjunctivitis

摘要

摘要:
目的了解2021年上海市B亚属人腺病毒分离株的基因特征。
方法对1例腺病毒55型感染导致的急性结膜炎病例进行病原体分离鉴定及全基因组测序，分析主要抗原蛋白六邻体（Hexon）、五邻体（Penton）、纤维突蛋白（Fiber）基因及全基因组序列的进化特征。
结果该病毒株属于B亚属B2亚组，Hexon基因与HAdV⁃11进化关系相近，而Fiber和Penton基因则与HAdV⁃14进化关系更为接近。核苷酸序列比对分析显示，该病毒与近期国内外流行的HAdV⁃55病毒株同源性均超过99.90%，与广东株73⁃GD_CHN_2016核苷酸相似度最高，在E1B、L4、E3和L5基因区存在氨基酸变异。
结论上海株（MH2021001）为腺病毒B亚属55型，该病毒基因组未发生多基因片段重组，与国内流行病毒株相比存在部分氨基酸变异，其生物学特性有待进一步研究。

Abstract:
Objective To determine the genomic characteristics of a subgenus B human adenovirus strain isolated in Shanghai in 2021.
Methods An adenovirus type 55 strain was isolated and identified from a patient with acute hemorrhagic conjunctivitis （AHC）. Complete genome of the strain was obtained using the next-generation sequencing （NGS）. Phylogenetic trees were reconstructed based on the sequences of Hexon， Fiber， Penton and complete genome to genomically characterize this strain.
Results Phylogenetic analysis based on the complete genome classified this strain （MH2021001） into subgenus B， subspecies B2 of HAdV-55. Hexon gene of MH2021001 had close phylogenetic relationship with HAdV-11， while Fiber and Penton genes had close relationship with HAdV-14. The MH2021001 showed high nucleotide identity with currently prevalent HAdV⁃55 strains （>99.90%）. The complete genome had 99.96% nucleotide identity to the 73-GD_CHN_2016 strain isolated in Guangdong. In addition， the amino acid sequence of MH2021001 had several substitutions in regions coding for E1B， L4， E3 and L5.
Conclusion This strain has been classified to HAdV-B55. No recombination event is identified in the complete genome. Due to multiple amino acid substitutions， the biological characteristics of the strain need to be further identified.

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