毛传武，张莉，臧金鑫，等.江苏省海门市某村生活用水相关水环境中抗生素抗性基因的分布［J］.上海预防医学，2023，35（12）：1199-1205.. doi: 10.19428/j.cnki.sjpm.2023.23158
引用本文: 毛传武，张莉，臧金鑫，等.江苏省海门市某村生活用水相关水环境中抗生素抗性基因的分布［J］.上海预防医学，2023，35（12）：1199-1205.. doi: 10.19428/j.cnki.sjpm.2023.23158
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MAO Chuanwu,ZHANG Li,ZANG Jinxin,et al.Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province[J].Shanghai Journal of Preventive Medicine,2023,35(12):1199-1205.. doi: 10.19428/j.cnki.sjpm.2023.23158
Citation: MAO Chuanwu,ZHANG Li,ZANG Jinxin,et al.Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province[J].Shanghai Journal of Preventive Medicine,2023,35(12):1199-1205.. doi: 10.19428/j.cnki.sjpm.2023.23158
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江苏省海门市某村生活用水相关水环境中抗生素抗性基因的分布

Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province

    摘要
  • 摘要:
    目的 抗生素抗性基因（ARGs）已经在全世界受到了广泛的重视，旨在探索江苏省海门某村水体中的细菌群落结构、抗生素抗性基因种类与水平，比较分析各水体环境中微生物物种分布与抗生素抗性基因分布的特点。
    方法 抽取江苏省海门市10户居民并采集周边相关水环境样本，共采集河水（4份样本）、岷沟水（9份样本）与井水（8份样本）水体样本进行宏基因组测序，利用MetaWRAP进行组装，使用综合抗生素耐药性数据库（CARD）对ARGs进行注释，使用R软件对结果进行分析。
    结果 各种水体中优势菌门为变形菌门，优势菌属为后口动物属、假单胞菌属、黄杆菌属与链霉菌属，注释的ARGs中以喹诺酮类、氨基糖苷类、大环内酯类及β⁃内酰胺类抗生素抗性基因为主。抗性基因相对丰度前4位为macB、ranA、evgS与txR，井水水体和岷沟水体的抗性基因的绝对丰度平均值与抗性基因表达高于河水水体。
    结论 井水、河水与岷沟水体中都检测到了不同程度的多种ARGs，且井水水体与岷沟水体中的抗性基因表达要高于河水水体，可能是由人类生产生活活动导致。

     

    Abstract:
    Objective Antibiotic resistance genes （ARGs） have received wide attention all over the world. The purpose of this study was to explore the bacterial community structure， the types and levels of antibiotic resistance genes in a water body in east China， and to compare and analyze the characteristics of microbial species distribution and antibiotic resistance gene distribution in various water environments.
    Methods A total of 10 households in Haimen City， Jiangsu Province were selected and their surrounding water environment samples were collected. 21 water samples including river water （4）， Mingou water （9） and well water （8） were collected for metagenomics sequencing， assembled with MetaWRAP， annotated with CARD database， and analyzed with R software.
    Results In various water bodies， the dominant bacteria phyla was Proteobacteria， the dominant bacteria genera were Deuterostomia， Pseudomonas， Flavobacteriales and Streptomycetaceae. The ARGs annotated were mainly composed of quinolones， aminoglycosides， macrolides and beta-lactams antibiotic resistance genes. The top four relative abundance of resistance genes were macB， RanA， evgS and TxR， The average absolute abundance and expression of resistance genes in well water and Mingou water were higher than those in river water.
    Conclusion Multiple ARGs are detected to varying degrees in well water， river water， and Mingou water bodies， and the expression of resistance genes in well water and Mingou water bodies is higher than that in river water bodies， possibly due to human production and living activities.

     

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