马铭君，黄佳璐，朱黎丹，等.男性感染HIV异性性行为者的肠道菌群特征与肝肾功能损伤的相关性［J］.上海预防医学，2023，35（12）：1192-1198，1211.. doi: 10.19428/j.cnki.sjpm.2023.23322
引用本文: 马铭君，黄佳璐，朱黎丹，等.男性感染HIV异性性行为者的肠道菌群特征与肝肾功能损伤的相关性［J］.上海预防医学，2023，35（12）：1192-1198，1211.. doi: 10.19428/j.cnki.sjpm.2023.23322
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MA Mingjun,HUANG Jialu,ZHU Lidan,et al.Correlation between intestinal flora characteristics and hepatic and renal impairments in HIV-infected heterosexual men[J].Shanghai Journal of Preventive Medicine,2023,35(12):1192-1198.. doi: 10.19428/j.cnki.sjpm.2023.23322
Citation: MA Mingjun,HUANG Jialu,ZHU Lidan,et al.Correlation between intestinal flora characteristics and hepatic and renal impairments in HIV-infected heterosexual men[J].Shanghai Journal of Preventive Medicine,2023,35(12):1192-1198.. doi: 10.19428/j.cnki.sjpm.2023.23322
shu

男性感染HIV异性性行为者的肠道菌群特征与肝肾功能损伤的相关性

Correlation between intestinal flora characteristics and hepatic and renal impairments in HIV-infected heterosexual men

    摘要
  • 摘要:
    目的 分析感染人类免疫缺陷病毒（HIV）的男性异性性行为者的肠道菌群特征及肝肾功能损伤情况，并探讨两者之间的相关性。
    方法 对41例男性感染HIV的异性性行为者（PMSW）及43例年龄和BMI相匹配的健康男性异性性行为者（NMSW）的粪便样本进行16S rDNA测序，测定外周血天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、总胆红素（TBIL）、尿素（UREA）、肌酐（Cr）、尿酸（UA）、β2微球蛋白（β2⁃MG）等肝肾功能指标的水平。采用生物信息学方法分析肠道菌群特征，比较差异菌种，并分析其与肝肾功能指标的相关性。
    结果 与NMSW相比，PMSW的肠道菌群α多样性降低，β多样性分析显示两组人群的组间菌群特征差异具有统计学意义（P<0.05）。梭菌、厚壁菌门、毛螺菌、瘤胃球菌丰度下降，梭杆菌丰度上升（线性判别分析LDA得分>4）。肝肾功能指标比较发现PMSW的AST、β2⁃MG水平高于NMSW，TBIL则低于NMSW。同时，PMSW的β2⁃MG异常人数远高于NMSW，差异具有统计学意义（P<0.001）。相关性分析发现AST与梭菌呈负相关（P<0.05）；TBIL与梭杆菌呈负相关（P<0.05），与厚壁菌门、毛螺菌呈正相关（P<0.05）；β2⁃MG与厚壁菌门、梭菌、毛螺菌、瘤胃球菌呈负相关（P<0.05），与梭杆菌呈正相关（P<0.05）。
    结论 PMSW的菌群α多样性降低；AST、β2⁃MG水平升高，TBIL水平降低且与差异菌种存在明显相关性。

     

    Abstract:
    Objective To explore the relationship between the intestinal flora and the impairment of liver and kidney in HIV-infected men who have heterosexual sex with healthy women.
    Methods Fecal samples from 41 HIV-infected heterosexual men who have sex with women （PMSW） and 43 age- and BMI-matched healthy heterosexual men who have sex with women （NMSW） were collected and subjected to 16S rDNA sequencing. The blood levels of AST， ALT， TBIL， UREA， Cr， UA， β2-MG and other liver and kidney function indicators were measured. Bioinformatics methods were used to analyze the characteristics of the intestinal flora of the patients in these two groups， to compare the differential bacteria strains， and to analyze their correlation with liver and kidney function indicators.
    Results In comparison with NMSW， the alpha diversity of intestinal flora was decreased in PMSW， and the beta diversity analysis showed significant differences in flora characteristics between the two groups （P<0.05）. The abundance of Clostridium， Phylum thick-walled， Trichosporon， and Clostridium tumefaciens decreased but Fusobacteriota increased （LDA score >4）. The comparison of liver and kidney function indexes revealed that AST， β2-MG levels were higher in PMSW than in NMSW， while TBIL was lower in PMSW than in NMSW. The number of patients with abnormal β2-MG was much higher in PMSW than in NMSW， and the difference was statistically significant （P<0.001）. It was also found that AST was negatively correlated with Clostridium （P<0.05）； TBIL was negatively correlated with Clostridium and positively correlated with Phylum thick-walled and Trichosporon （P<0.05）. β2-MG was negatively correlated with Phylum thick-walled， Clostridium， Trichosporon and Rumenococcus （P<0.05） and positively correlated with Clostridium （P<0.05）.
    Conclusion In PMSW group， the alpha diversity of the flora is decreased. AST and β2-MG levels are increased， and TBIL level is decreased. These changes were significantly correlated with different strains of bacteria in the intestinal flora.

     

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