Abstract: Objective To analyze the epidemic characteristics of AIDS, gonorrhea and syphilis from 1990 to 2013 in Huangpu District, Shanghai, and to evaluate the effects of prevention and control in this regard. Methods Ecology research method was used to analyze epidemic characteristics of sexually transmitted disease. Results From 1990 to 2013, the incidence of gonorrhea increased first and then decreased in recent years, while that of syphilis had been increasing since 1994, among which latent syphilis was the most. AIDS cases occurred each year from 2006. The young male adults of 25-39 years old were the main population who had these three kinds of sexually transmitted diseases. The cases of AIDS and syphilis were mainly found in those engaged in housekeeping, housework and the unemployed, and gonorrhea was mostly with workers. Conclusion The incidence of gonorrhea in Huangpu District has been decreasing, while the prevention and control of AIDS and syphilis still needs to be strengthened. The incidence is influenced by the change of people's awareness and behavior. In future, we should focus on key groups like young males, housekeeping, unemployed and recurrent population.