Abstract: Objective To evaluate the immune effect of hepatitis B vaccine by different inoculating programs for adults as 0-1-3 month, 0-1-6 month and 0-1-12 month and to provide evidence for the development of adult immunization strategy. Methods In Tongxiang City of Zhejiang Province, 10μg of hepatitis B vaccine was voluntarily inoculated by different immunization programs as 0-1-3 month, 0-1-6 month or 0-1-12 month among adults aged 16 to 49 years. Quantitative detection of anti-HBs was conducted after 1 month and 1 year of immunization, and the positive seroconversion rates and geometric mean titers of anti-HBs (GMT) were evaluated. Results Totally 682 subjects were evaluated. The anti-HBs positive rates were 99.85% after 1 month of immunization, and 70.23% after 1 year of immunization. It was observed that GMT of anti-HBs was higher with adults of younger age. Conclusion Adults can achieve better immune effect by inoculation of 10μg of hepatitis B vaccine and when it is done in target population according to 0-1-6 month program,compliance is better and immune effect proves more persistent. It is suggested that hepatitis B vaccine should be inoculated for the adults as early as possible to ensure the effect and persistence of the vaccine.