Abstract: Objective To investigate the prevalence and causes of gallstone in permanent residents in Chenguan District of Lhasa City. Methods By cluster sampling, 1200 permanent residents were investigated by interview, Telephone and self-administered questionnaire about their sex, age, ethnic, diet habit, life behavior, mental status and so on.All the data were analyzed using SPSS 16.0 software. Results The investigation showed that the prevalence rate of gallstone in this area was 23.0%.There was a close relationship between the formation of gallstone and the sex, occupation, life style and diet habit, disease factors. Conclusion The formation of gallstone was found to be the comprehensive results of multifactor, which were mainly related to sex, occupation, diet habits, diseases while there was no significant relationship found with ethnic relations in this area.