Abstract: Objective To ascertain the serotype and the drug resistance of Vibrio parahaemolyticus in Pudong New Area of Shanghai.Methods The slide serum agglutination test was used to detect the 160 serotypes of Vibrio parahaemolyticus and the K-B method was used to test the drug resistance.Results In 59 strains isolated from food poisoning，7 serotypes were typed in 56 strains. The total typing rate was 94.92% and the main serotype was O3∶K6 accounting for 77.97%. In 101 strains isolated from aquatic products, 22 serotypes with no popular type were typed in 32 strains and the total typing rate was 31.68%.The test of drug resistance showed high resistance to Ampicillin and 100.00% sensitive to Amoxicillin/clavulanic acid, Cefotaxime, Ceftazidime, Cefepime and Chloramphenicol.Conclusion The main serotype of Vibrio parahaemolyticus from food poisoning strains in Pudong was O3∶K6. The serotypes isolated from aquatic products were diverse and different from the serotypes of food poisoning strains. Vibrio parahaemolyticus in Pudong did not mutate widely in drug resistance test.