Abstract: Objective To establish a method for determination of hydroxybenzene in drinking water by flow injection spectrophotometry.Methods Hydroxybenzene in drinking water was determined by flow injection spectrophotometry, and the best experimental conditions were chosen for that purpose.Results In the best conditions, the linear range was 2.0-200.0 μg/L, the detection limit of the method was 0.9 μg/L, RSD of the determination of standard solution was 1.42%.The method was applied for analysis of drinking water, with satisfactory results obtained, and the average recovery was 94.5%-112.4%.The samples were determined by the proposed method and standard method.The results obtained showed no significant difference from those achieved by the international method.Conclusion This method is accurate and reproducible, suitable for the determination of hydroxybenzene in drinking water.