Primary drug resistance of newly diagnosed HIV-1 infected men who have sex with men in Changning District of Shanghai, 2014-2017

ObjectiveTo investigate the characteristics, subtype and primary drug resistance among HIV-1 infected men who have sex with men (MSM) in Changning District of Shanghai from 2014 to 2017, and to provide evidence for antiretroviral therapy(ART).

MethodsA total of 363 newly diagnosed HIV-1 positive MSM were chosen from 2014 to 2017 with ART free to analysis HIV-1 subtype and primary drug resistance via reverse transcription-polymerase chain reaction (RT-PCR) and sequencing.

ResultsThe 300 pol gene fragments were finally obtained from RT-PCR.These cases were aged from 18 to 78 years; 212 of them (71.7%) came from other provinces; 165(55.0%) had education level of college or above; 71.7% were unmarried; 154(51.3%) were with the subtype of CRF01_AE; 104(34.7%) with the subtype of CRF07_BC and 42(14.0%) with the subtype of other recombinants.Eighteen cases (6.0%) had the primary drug resistance mutations.The mutation associated drug resistant rates to non-nucleoside reverse transcriptase inhibitors(NNRTI), nucleoside reverse transcriptase inhibitors(NRTI) and protease inhibitors(PI) were 4.3%(13/300), 2.7%(8/300) and 1.3%(4/300), respectively; 2.0%(6/300) of the cases owned two or more resistance mutations at the same time.

ConclusionCRF01_AE is the major subtype among HIV-1 infected MSM in Changning District, Shanghai, and the primary drug resistance mutation associated with NNRTI is relatively high, indicating that more attention should be paid to the use of NNRTI in clinic and more powerful surveillance for MSM primary drug resistance needed.