ObjectiveTo verify the limit of detection as well as sensitivity and specificity of real-time isothermal detection and rapid detection kit of Vibrio parahaemolyticus(VP).
Methods By detecting three groups of samples- laboratory prepared samples, commercial food samples and diarrhea patients samples, the stability and reliability as well as the scope of application of the method and kit were verified.
Results Laboratory configuration sample detection showed a stable and reliable result, in which the detection limit reached 7 to 100 cfu/mL.Although the FPR of food sample and diarrhea patients sample were relatively high, the sensitivity was great.The total sensitivity, specificity, true positive rate, true negative rate were respectively 99.6%, 55.9%, 85.1%, 98.3%.
Conclusion The real-time isothermal detection and rapid detection kit have a great sensitivity for VP detection.Also the results are stable and essentially in agreement with the culture method of GB 4789.7—2013. The method is worth being promoted in application, under the premise that experimental operating steps and experimental conditions are well-controlled in order to improve the specificity.
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