Oil-red O special staining techniques applied in fatty liver tissue of hyperlipidemic mouse

ObjectiveTo explore the application of two oil-red staining methods for steatosis in non-alcoholic fatty liver disease.

MethodsThirty C57BL/6J mice models of non-alcoholic fatty disease were selected. After euthanasia, the left liver lobe was harvested immediately, which was immersed in a large volume of 4% paraformaldehyde fixative in cacodylate buffer. After fixation, the liver was divided into two samples for making histological sections. One sample was made into paraffin sections for hematoxyin-eosin (HE) staining to identify steatosis. The other was made into frozen sections of OCT-embedded block after dehydration with 30% sucrose solution. The frozen sections were stained by two oil-red O staining methods. The localization of oil-red O in the lipid droplet was observed by blind measurement under microscope, and the results obtained by the two staining methods were compared.

ResultsThe two staining methods proved to be without pathological damage to tissue and cell, and had good coloration and localization effect, which provided scientific basis for the diagnosis of non-alcoholic fatty liver disease.

ConclusionBy the use of good special staining technology, the lipid drop cells are distinguished clearly, and the number of lipid drop cells are displayed. The steatosis in non-alcoholic fatty disease is located and analyzed with half quantification.