Guang-rui JIAO, Ke WANG. Simultaneous determination of 4 carotenoid pigments in yolk by liquid chromatography-tandem mass spectrometry[J]. Shanghai Journal of Preventive Medicine, 2018, 30(6): 472-475. DOI: 10.19428/j.cnki.sjpm.2018.18814
Citation: Guang-rui JIAO, Ke WANG. Simultaneous determination of 4 carotenoid pigments in yolk by liquid chromatography-tandem mass spectrometry[J]. Shanghai Journal of Preventive Medicine, 2018, 30(6): 472-475. DOI: 10.19428/j.cnki.sjpm.2018.18814

Simultaneous determination of 4 carotenoid pigments in yolk by liquid chromatography-tandem mass spectrometry

  • ObjectiveTo establish a method for simultaneous determination of four carotenoid pigments (canzthaxanthin, citranaxanthin, β-apo-8-carotenoic acid ethyl ester, β-apo-8-carotenal) in yolk by liquid chromatography-tandem mass spectrometry (LC/MS/MS).
    MethodsYolk was extracted by acetonitrile, and then separated by Waters Xterra MS C18 column.Mass spectrometry used electrospray ionization positive ionization mode(ESI+) and multiple reaction monitoring(MRM) for determination with external standard method for quantification.
    ResultsThe detection limit of the 4 carotenoid pigments were 2.5 ng/g.The method showed a good linearity over the range of 2.5~200 ng/g with r > 0.99.The average recoveries were in the range of 82.24%~109.2%, while the relative standard deviations (RSD) were 1.0%~3.0% (n=6).
    ConclusionThe established method is highly sensitive and accurate, which is suitable for the detection of four carotenoid pigments in yolk.
  • loading

Catalog

    Turn off MathJax
    Article Contents

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return