FENG Rui, HU Qing, SUN Jian, YU Hong, ZHANG Su, ZHANG Jing-xian, SHEN Ying-ying, ZHU Yan-rong, WANG Ke, JI Shen. Determination of 30 illegally added drugs of disgestive regulation in foods by UHPLC-QQQ-MS[J]. Shanghai Journal of Preventive Medicine, 2019, 31(6): 432-438. DOI: 10.19428/j.cnki.sjpm.2019.19479
Citation: FENG Rui, HU Qing, SUN Jian, YU Hong, ZHANG Su, ZHANG Jing-xian, SHEN Ying-ying, ZHU Yan-rong, WANG Ke, JI Shen. Determination of 30 illegally added drugs of disgestive regulation in foods by UHPLC-QQQ-MS[J]. Shanghai Journal of Preventive Medicine, 2019, 31(6): 432-438. DOI: 10.19428/j.cnki.sjpm.2019.19479

Determination of 30 illegally added drugs of disgestive regulation in foods by UHPLC-QQQ-MS

  • ObjectiveAn analytical method was developed for the determination of 30 digestive system drugs in foods and dietary supplements by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QQQ-MS).
    MethodsA Waters CORTECS T3 column (100 mm ×2.1 mm, 2.7 μm) was used with acetonitrile and 0.1% formic acid aqueous solution as mobile phases by gradient elution for separation of 30 compounds.All the compounds were detected by electrospray ion souce in positive mode with multiple reactions monitoring (MRM) mode.
    ResultsThe calibration curves showed good linearities for the 30 compounds during the range of 1-10 μg/L.The correlation coefficients of the standard calibration curves for the 30 compounds were all above 0.99.The recoveries of the drugs at spiked levels of limits of quantitation (LOQs), double LOQs, ten times of LOQs were at the range of 80.1%~116.6% with the RSDs between 0.7% and 8.8% (n=6), except for the 5 compounds such as mesalazine with strong matrix effects.The LODs were 25 μg/kg for the 30 compounds.
    ConclusionThe method proves to be sensitive and accurate for the determination of the digestive system drugs.This method fills the gap of the determination of the digestive system drugs, which strikes illegal adulteration behavior effectively.
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