A method for examination of L-carnosine， eyeseryl，glutathione and acetyl hexapeptide-8 in cosmetics by HPLC

ObjectiveTo establish a HPLC method for examination of L-carnosine，eyeseryl，glutathione and acetyl hexapeptide-8 in cosmetics.

MethodAfter being extracted by water， L-carnosine， Eyeseryl， Glutathione， and Acetyl Hexapeptide-8 were examined by HPLC with methanol-0.01% formic acid （V/V） aqueous solution as the mobile phase. The column was Agilent Eclipse XDB-C18 （5 μm， 4.6 mm×150 mm）. The flow rate was 0.5 mL/min. The determination wavelength was set at 210 nm.

ResultsThere was a good linear relationship within the range of 5-100 μg/mL for L-carnosine， eyeseryl， glutathione， and acetyl hexapeptide-8. The recoveries of L-carnosine Eyeseryl Glutathione and Acetyl Hexapeptide-8 were between 92.5%~105.9%， with a RSD from 0.5% to 3.5%.

ConclusionThe method is simple， sensitive， specific and reproducible in the examination of L-carnosine， Eyeseryl， Glutathione， and Acetyl Hexapeptide-8 in cosmetics.