CUI Xiao-xian, MOU Jia-bin, TENG Zheng, ZHOU Yan-qiu, FANG Fang-hao, CHEN Hong-you, JIANG Hui, LI Chong-shan, CHEN Min, ZHANG Xi. Study on the stability of SARS-CoV-2 at different temperatures[J]. Shanghai Journal of Preventive Medicine, 2021, 33(9): 818-823. DOI: 10.19428/j.cnki.sjpm.2021.20498
Citation: CUI Xiao-xian, MOU Jia-bin, TENG Zheng, ZHOU Yan-qiu, FANG Fang-hao, CHEN Hong-you, JIANG Hui, LI Chong-shan, CHEN Min, ZHANG Xi. Study on the stability of SARS-CoV-2 at different temperatures[J]. Shanghai Journal of Preventive Medicine, 2021, 33(9): 818-823. DOI: 10.19428/j.cnki.sjpm.2021.20498
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Study on the stability of SARS-CoV-2 at different temperatures

    Abstract
  • ObjectiveTo observe the stability of severe acute respiratory syrdrome coronavirus （SARS-CoV-2） in cell cultures at different temperatures so as to provide basic data and scientific basis for the research and control of COVID-19 epidemic.
    MethodsThe Vero E6 cells inoculated with SARS-CoV-2. According to TCID50， SARS-CoV-2 with different dilution （10-1， 10-3， 10-5， 10-6）were stored at 37 °C， 22.5 °C， and 4 °C for one to seven days， and then infectious titer was determined by micro cytopathogenic effect assay， observing cytopathic effect （CPE）， and real-time fluorescence quantitative testing.
    ResultsSARS-CoV-2 was stable under 4 °C. The infectivity of high concentration （10-1 dilution） under 22.5 °C for seven days gradually decreased， while lower concentration completely lost infectivity after one day. The virus lost infectivity when stored at 37 °C for more than one day.
    ConclusionSARS-CoV-2 is highly stable at 4 °C， sensitive to heat， and related to virus concentration.
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