ZHU Chuan-xin, ZHENG Wen-li, WU Mao-mao, ZHENG Yan-rong, XU Chang-ping. Rapid screening of Salmonella by loop-mediated isothermal amplification combined with lateral flow dipstick[J]. Shanghai Journal of Preventive Medicine, 2021, 33(6): 492-495. DOI: 10.19428/j.cnki.sjpm.2021.20719
Citation: ZHU Chuan-xin, ZHENG Wen-li, WU Mao-mao, ZHENG Yan-rong, XU Chang-ping. Rapid screening of Salmonella by loop-mediated isothermal amplification combined with lateral flow dipstick[J]. Shanghai Journal of Preventive Medicine, 2021, 33(6): 492-495. DOI: 10.19428/j.cnki.sjpm.2021.20719
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Rapid screening of Salmonella by loop-mediated isothermal amplification combined with lateral flow dipstick

    Abstract
  • ObjectiveTo establish a specific and sensitive method using loop-mediated isothermal amplification for rapid screening of Salmonella.
    MethodsThe invA gene sequence of Salmonella was downloaded from GenBank. After homology comparison with DNAMAN software， amplification primers were designed in the conserved region， and a LAMP-LFD detection method was established. The reaction system was optimized， and the specificity and sensitivity of the method were verified.
    ResultsThe sensitivity of this method to detect Salmonella DNA was up to 1.0×101 copies/μL. The positive rate of anal swabs was the same as that of fluorescent PCR. Meanwhile， LAMP-LFD was easy to operate and did not need expensive instruments. The detection result could be obtained within 30 minutes.
    ConclusionThe LAMP-LFD method established in this study is rapid， simple， sensitive and specific， which is suitable for rapid screening of Salmonella.
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