Kinetic turbidmetric limulus test for quantative determination of bacterial endotoxin in human prothrombin complex

Objective To determine bacterial endotoxin quantitatively in human prothrombin complex by kinetic turbidimetric limulus test.Methods The validation was investigated, when the amount of endotoxin spiked in the sample were recovered within the range 50%-200%.Results Human prothrombin complex did not interfere in limulus test.Conclusion The result by kinetic turbidimetric limulus test was consistent with that of the rabbit pyrogen test. The kinetic turbidimetric limulus test is suitable for quantitative determination of endotoxin in human prothrombin complex.