连宪强, 杨粤, 王文祥, 张建波. 深圳市大鹏新区首发登革热病例的分子病毒学分析[J]. 上海预防医学, 2021, 33(6): 467-470. DOI: 10.19428/j.cnki.sjpm.2021.20023
引用本文: 连宪强, 杨粤, 王文祥, 张建波. 深圳市大鹏新区首发登革热病例的分子病毒学分析[J]. 上海预防医学, 2021, 33(6): 467-470. DOI: 10.19428/j.cnki.sjpm.2021.20023
LIAN Xian-qiang, YANG Yue, WANG Wen-xiang, ZHANG Jian-bo. Molecular analysis of the first dengue fever case in Dapeng New District of Shenzhen City[J]. Shanghai Journal of Preventive Medicine, 2021, 33(6): 467-470. DOI: 10.19428/j.cnki.sjpm.2021.20023
Citation: LIAN Xian-qiang, YANG Yue, WANG Wen-xiang, ZHANG Jian-bo. Molecular analysis of the first dengue fever case in Dapeng New District of Shenzhen City[J]. Shanghai Journal of Preventive Medicine, 2021, 33(6): 467-470. DOI: 10.19428/j.cnki.sjpm.2021.20023

深圳市大鹏新区首发登革热病例的分子病毒学分析

Molecular analysis of the first dengue fever case in Dapeng New District of Shenzhen City

  • 摘要:
    目的对深圳市大鹏新区首例疑似登革热病例的病原体进行分析及型别鉴定,从分子水平对病毒的生物学特征进行研究,以追溯病毒的地域来源。
    方法采用实时荧光RT-PCR方法对采集的疑似登革热患者血液样本进行登革病毒核酸检测及分型鉴定。扩增E基因并进行序列测定,与国内外不同地区登革热病毒株进行同源性比较和遗传进化分析。
    结果疑似登革热患者血清中检出登革1型病毒核酸。系统发育树显示分离株DP-DGR19001与MG894965/TW/CHN/2016、LC428079/VNM/2017、MG894867/TW/CHN/2015及MN512242/GX/CHN/2014的亲缘关系较近,属于genotype Ⅰ型。E基因核苷酸序列同源性分别为99.87%、99.80%、99.60%和99.46%,氨基酸序列同源性均为100%。患者在发病前14 d内有前往登革热高发区的旅行史。
    结论大鹏新区首发登革热病例为登革1型病毒感染,属于输入性病例。

     

    Abstract:
    ObjectiveTo analyze the first suspected dengue fever case in Dapeng New District by characterizing the virus and exploring its origin.
    MethodsDengue virus nucleic acid in blood sample was identified by real-time fluorescent RT-PCR. The E gene of dengue virus was amplified and sequenced. Homology and phylogenetic tree of this dengue virus were compared with the isolates from other regions.
    ResultsNucleic acid of dengue virus type 1 was detected in the serum sample from the suspected dengue fever patient. Phylogenetic tree showed that the homology based on nucleotide sequence of E gene of DP-DGR19001 was very similar with the isolates of MG894965/TW/CHN/2016 (99.87%), LC428079/VNM/2017(99.80%), MG894867/TW/CHN/2015 (99.60%) and MN512242/GX/CHN/2014 (99.46%). The deduced amino acid sequence of 5 isolates was 100% identical. The patient had traveled to high-incidence country Cambodia in 14 days before infection.
    ConclusionsThe first suspected dengue fever case in Dapeng New District is caused by dengue virus type 1. The case is an imported dengue fever .

     

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