黄妙芬, 王文祥, 连宪强, 杨粤. 深圳市大鹏新区2起儿童疱疹性咽颊炎聚集性疫情的病原鉴定与基因分型分析[J]. 上海预防医学, 2022, 34(7): 660-664. DOI: 10.19428/j.cnki.sjpm.2022.21630
引用本文: 黄妙芬, 王文祥, 连宪强, 杨粤. 深圳市大鹏新区2起儿童疱疹性咽颊炎聚集性疫情的病原鉴定与基因分型分析[J]. 上海预防医学, 2022, 34(7): 660-664. DOI: 10.19428/j.cnki.sjpm.2022.21630
HUANG Miaofen, WANG Wenxiang, LIAN Xianqiang, YANG Yue. Pathogen identification and genotyping of two cluster outbreaks of herpangina in children in Dapeng New District, Shenzhen[J]. Shanghai Journal of Preventive Medicine, 2022, 34(7): 660-664. DOI: 10.19428/j.cnki.sjpm.2022.21630
Citation: HUANG Miaofen, WANG Wenxiang, LIAN Xianqiang, YANG Yue. Pathogen identification and genotyping of two cluster outbreaks of herpangina in children in Dapeng New District, Shenzhen[J]. Shanghai Journal of Preventive Medicine, 2022, 34(7): 660-664. DOI: 10.19428/j.cnki.sjpm.2022.21630

深圳市大鹏新区2起儿童疱疹性咽颊炎聚集性疫情的病原鉴定与基因分型分析

Pathogen identification and genotyping of two cluster outbreaks of herpangina in children in Dapeng New District, Shenzhen

  • 摘要:
    目的 分析2021年5月深圳市大鹏新区2起儿童疱疹性咽颊炎疫情的病原特征及其基因型。
    方法 采集2起疱疹性咽峡炎暴发疫情患儿的5份咽拭子,用实时荧光聚合酶链式反应(PCR)对其进行常见肠道病毒的基因检测,采用逆转录巢式聚合酶链式反应(RT⁃nPCR)法扩增肠道病毒VP1区,通过测序和比对进行序列分析,运用MEGA7软件中的CLUSTAL W程序进行位点对齐并构建系统进化树。
    结果 引起这2起疱疹性咽峡炎疫情的病原体为柯萨奇病毒A4(CVA4)。VP1区基因序列分析发现2起疫情样本之间的核苷酸同源性为92%;A幼儿园的3株毒株与深圳2018株(MN840533)进化关系最近,核苷酸同源性为98.11%;B幼儿园的2株毒株与四川2018株(MW178763)进化关系最近,核苷酸同源性为97.88%。系统进化树显示,本研究的5株CVA4病毒均属C2基因亚型。
    结论 2起儿童疱疹性咽颊炎疫情都是由CVA4病毒引起,均属C2基因亚型。

     

    Abstract:
    Objective To determine the pathogenic characteristics and genotype of two outbreaks of herpangina in children in Dapeng New District, Shenzhen, in May 2021.
    Methods A total of five throat swabs from children in the two outbreaks of herpangina were collected and examined for common enteroviruses by real-time PCR. The VP1 region was further amplified by nested RT-PCR. The CLUSTAL W program in MEGA7 software was used to conduct the alignment and reconstruct a phylogenetic tree.
    Results The pathogen causing the 2 cluster outbreaks of herpangina was coxsackievirus A4 (CVA4). The sequences of CVA4 VP1 genes revealed that a nucleotide identity of 92% between the strains in the two outbreaks. The three CVA4 strains isolated in kindergarten A had the closest phylogenetic relationship with that isolated in Shenzhen in 2018(MN840533), with the nucleotide identity of 98.11%. The two strains in kindergarten B had the closest phylogenetic relationship with CVA4 strain isolated in Sichuan in 2018(MW178763), with the nucleotide identity of 97.88%. The phylogenetic tree showed that all five CVA4 strains in this study belonged to the C2 genotype.
    Conclusion The C2 genotype of CVA4 is the causative agent in both outbreaks of herpangina.

     

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