长链非编码<b>RNA Linc‑pint</b>通过调控微<b>RNA‑21</b>介导结直肠癌进程的机制研究

李梦成; 丁呈圣; 刘立果; 单泽志; 金志明

doi:10.19428/j.cnki.sjpm.2022.22010

长链非编码RNA Linc‑pint通过调控微RNA‑21介导结直肠癌进程的机制研究

Long non‑coding RNA Linc‑pint mediates the progression of colorectal cancer by regulating miRNA‑21

摘要

摘要:
目的研究长链非编码RNA Linc‑pint在结直肠癌（CRC）中的生物学作用及其分子机制。
方法通过实时定量聚合酶链反应（qRT‑PCR）检测Linc‑pint在31对CRC组织及其癌旁正常组织中的表达水平。分析Linc‑pint的表达与患者临床病理特征之间的关系；采用Kaplan‑Meier生存分析法分析Linc‑pint表达与患者预后的关系，Cox回归模型分析各项临床病理特征与患者预后的关系。采用qRT‑PCR检测5种常见CRC细胞株中Linc‑pint表达水平，通过细胞增殖实验、Transwell侵袭和迁移实验、裸鼠皮下成瘤实验检测Linc‑pint对细胞增殖、侵袭及迁移能力的影响。通过qRT‑PCR检测Linc‑pint的靶基因微RNA（miR）‑21在31对CRC组织及其癌旁组织中的表达，并利用皮尔森相关系数分析两者之间的相关性。在CRC细胞中过表达Linc‑pint后采用qRT‑PCR检测其对miR‑21表达的影响。
结果 Linc‑pint在癌旁正常组织中表达水平为3.95±1.16，高于CRC组织的2.74±0.95（t=6.17，P<0.05）。Linc‑pint高表达CRC患者的总体生存率为62.5%（30/48），高于Linc‑pint低表达CRC患者的34.3%（24/70），差异有统计学意义（P<0.05）。过表达Linc‑pint后，CRC细胞增殖、侵袭及迁移能力均被抑制（均P<0.05）。在肿瘤组织和癌旁正常组织中，Linc‑pint表达水平与miR‑21表达水平呈负相关（r=-0.288、-0.908，均P<0.05）。
结论 Linc‑pint可通过作用于miR‑21介导结直肠癌的增殖、侵袭和迁移。

Abstract:
Objective To investigate the biological function and molecular mechanism of long non-coding RNA Linc‑pint in colorectal cancer.
Methods Quantitative real‑time quantitative （qRT‑PCR） was performed to detect the expression level of Linc‑pint in 31 pairs of colorectal cancer tumor and adjacent normal tissues； correlation between the expression level of Linc‑pint and the clinicopathological characteristics was analyzed by the chi‑square test. Kaplan-Meier survival analysis was used to assess the relationship between Linc‑pint expression level and the prognosis of patients. Cox regression model was used to analyze the relationship between clinicopathological characteristics and the prognosis of patients. Expression level of Linc‑pint were detected by qRT‑PCR in 5 common colorectal cancer cell lines. Effect of Linc‑pint on cell proliferation， invasion and migration was measured by cell counting kit‑8 assay， Transwell assay and harvested xenografts from nude mice. qRT‑PCR was performed to detect the expression level of Linc‑pint's target gene micro RNA（miR）‑21 in 31 pairs of colorectal cancer tumor tissues and adjacent normal tissues. Pearson correlation coefficient was used to assess the correlation between Linc‑pint and miR‑21. qRT‑PCR was used to detect the expression of overexpression of Linc‑pint on miR‑21 in colorectal cancer cells.
Results Expression level of Linc‑pint in normal tissues （3.95±1.16） was significantly higher than that in colorectal cancer tissues （2.74±0.95）（t=6.17， P<0.05）. Overall survival rate of patients with high expression of Linc‑pint was 62.5%， which was significantly higher than that of patients with low expression of Linc‑pint （34.3%， P<0.05）. The proliferation， invasion and migration of CRC cells were inhibited after overexpression of Linc‑pint. In colorectal cancer tumor and adjacent normal tissues， Linc‑pint and miR‑21 showed opposite expression in tumor tissues and were negatively correlated （r=-0.288 and -0.908， both P<0.05）.
Conclusion Linc‑pint acts as a tumor suppressor by down‑regulating the expression level of miR‑21 to inhibit the proliferation， invasion and migration of colorectal cancer.

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