Abstract: To sort out the requirements for enhancing the capacity and informatization construction of CDC laboratories in the context of high-quality development of disease prevention and control. To investigate and analyze the current situation, problems faced and development needs for laboratory informatization construction of disease prevention and control institutions in Shanghai, so as to provide an evidence for putting forward targeted suggestions. In addition, it will provide ideas for comprehensively improving the informatization construction of laboratories in disease prevention and control institutions and constructing a smart and accurate modern disease prevention and control system under the new context.