围产期双酚<b>AF</b>暴露对子代雄性大鼠青春前期的致肥作用

刘玺; 杨正丽; 成雪晴; 杨隽; 徐菁; 洪新宇

doi:10.19428/j.cnki.sjpm.2026.250256

围产期双酚AF暴露对子代雄性大鼠青春前期的致肥作用

Obesogenic effects of perinatal bisphenol AF exposure on prepubertal male offspring rats

摘要

摘要:
目的探究围产期暴露于环境内分泌干扰物双酚AF（BPAF）对子代雄性大鼠青春前期（出生后第21天，PND21）肝脏脂质代谢的干扰作用，旨在为评估BPAF的致肥作用提供科学依据。
方法研究采用8周龄SD大鼠，设BPAF剂量组（2、10、50 mg·kg^-1）和溶剂对照组（玉米油），每组6只确认受孕的雌鼠。从孕第0天起灌胃至哺乳期结束。PND21时每窝随机选1只雄性子鼠，检测血清中葡萄糖（GLU）、甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL⁃C）、低密度脂蛋白胆固醇（LDL⁃C）、瘦素（LEP）、游离脂肪酸（FFA）、氧化应激标志物超氧化物歧化酶（SOD）和丙二醛（MDA）浓度，观察肝脏和脂肪组织病理变化，检测肝脏脂肪代谢相关基因表达水平。
结果与溶剂对照组相比，50 mg·kg^-1组孕鼠的子代雄鼠血清LEP和MDA升高（P<0.05），肝脏脂蛋白脂肪酶（Lpl）、脂肪酸合成酶（Fas）和过氧化物酶体增殖物激活受体γ（Pparg）基因表达上调（P<0.05）；2 mg·kg^-1组脂肪细胞长度增加（P<0.05），50 mg·kg^-1组脂肪细胞面积与长度均增大（P<0.05）。肝脏组织病理学检查未见明显异常。
结论围产期50 mg·kg^-1 BPAF暴露可诱导青春前期子代雄鼠脂肪细胞肥大，瘦素水平升高，脂质合成基因表达上调及氧化应激增强，提示BPAF可能通过干扰脂质代谢通路发挥环境致肥作用。

Abstract:
Objective To investigate the disruptive effects of perinatal exposure to the environmental endocrine disruptor bisphenol AF (BPAF) on hepatic lipid metabolism in prepubertal (postnatal day 21, PND21) male offspring rats, and to provide scientific evidence for assessing the obesogenic effect of BPAF.
Methods Sprague-Dawley (SD) rats aged 8 weeks were used in this study. Pregnant rats were divided into BPAF dose groups (2, 10, 50 mg·kg⁻¹) and a vehicle control group (corn oil), with 6 confirmed pregnant females per group. Gavage administration started from gestational day 0 and continued until the end of lactation. At PND21, one male offspring per litter was randomly selected. Serum concentrations of glucose (GLU), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), leptin (LEP), free fatty acid (FFA), as well as oxidative stress markers superoxide dismutase (SOD) and malondialdehyde (MDA), were measured. Pathological changes in liver and adipose tissues were evaluated, and the expression levels of genes related to hepatic lipid metabolism were measured.
Results Compared to the vehicle control group, the 50 mg·kg⁻¹ group showed significantly increased serum LEP and MDA levels in male offspring (P<0.05), and significant upregulation of hepatic lipoprotein lipase (Lpl), fatty acid synthetase (Fas), and peroxisome proliferator-activated receptor γ (Pparg) gene expression (P<0.05). The 2 mg·kg⁻¹ group exhibited a significant increase in adipocyte length (P<0.05), while the 50 mg·kg⁻¹ group showed significant increases in both adipocyte area and length (P<0.05). No significant abnormalities were observed in liver histopathological examination.
Conclusion Perinatal exposure to 50 mg·kg⁻¹ BPAF induced adipocyte hypertrophy, elevated leptin levels, upregulation of lipid synthesis gene expression, and enhanced oxidative stress in prepubertal male offspring, suggesting that BPAF may exert environmental obesogenic effects by disrupting lipid metabolism pathways.

HTML全文

参考文献(27)

施引文献

资源附件(0)