Abstract: Objective To analyze the epidemiological and clinical characteristics of the 21 confirmed monkeypox cases in a district of Chengdu city, and to provide scientific guidance for the prevention and control of subsequent monkeypox epidemics. Methods Data of confirmed monkeypox cases with current addresses in this district were collected from the Disease Control and Prevention Information System of China. A retrospective descriptive epidemiological analysis was used to analyze the demographic, distributional and behavioral characteristics of the cases. Results Since the first confirmed case of monkeypox was reported on July 4, 2023, up to April 30, 2025, a total of 21 confirmed cases of monkeypox have been reported. All cases were male, with a mean age of 30.9 (30.9 ± 6.2) years. The majority were men who have sex with men(MSM) population (90.48%). 19.05% of cases were co-infected with HIV, and 19.05% had a history of syphilis infection. Within 21 days prior to symptom onset, 19 cases (90.48%) self-reported engaging in male-to-male sexual contact, among whom 10 cases (52.63%) reported having taken protective measures, while 9 cases (47.37%) did not take safety precautions. 13 cases (61.90%) had no travel history to area with reported monkeypox case during the 21 days before symptom onset.The predominant manifestation was exanthem (100%), followed by fever (57.14%), lymphadenectasis (47.62%), and muscle soreness (14.29%). Fever was predominantly low-grade (37.3–38℃), accounting for 50.00% of the cases. All cases were identified through active health-care seeking behavior. The median interval from symptom onset to the first visit to the hospital was 3.0 (2.0, 6.0) days, with a maximum interval of 14 days. The median interval from symptom onset to laboratory confirmation was 7.0(5.0，10.0) days. Six cases(28.57%) required two or more visits to the hospital, with bacterial infection being the primary initial misdiagnosis. Conclusion Monkeypox prevention and control efforts should prioritize men who have sex with men and young- to-middle-aged populations. It is recommended to establish an integrated monkeypox epidemic prevention and control network by leveraging existing HIV/AIDS prevention and control network. Concurrently, accelerating the deployment of the national intelligent infectious disease monitoring and early warning software to strengthen detection capabilities, which will be beneficial for the overall effectiveness of epidemic prevention and control efforts.