Abstract: 0bjective To describe the disposal process of the first imported case of Zika virus disease in Shanghai, and to provide a reference for the prevention and control of imported infectious diseases in the future. Methods The case was promptly identified through the entry surveillance system at customs, and the Center for Disease Control and Prevention (CDC) of Shanghai city and districts carried out the epidemiological investigation and pathogen detection. Results On April 8th, 2025, customs authority at a certain airport in Shanghai identified a feverish inbound case (case A,female, 40 years old) and collected cubitus vein blood sample to conduct nucleic acid tests for various mosquito-borne infectious diseases. The result showed that the Zika virus nucleic acid was positive. On April 9th, the CDC collected cubitus vein blood samples from the case A and three traveling companions to conduct nucleic acid tests for various arthropod-borne infectious diseases. The result showed that the Zika virus nucleic acid of case A was positive, and the dengue virus nucleic acid of companion D (male, 6 years old) was positive, while the other two companions were negative. Subsequently, case A and D were transferred to a designated hospital for isolation and treatment. Two patients left the hospital and departed the country to return to their overseas place of residence. Conclusion Case A was the first imported case of Zika virus disease in Shanghai. For patients with a history of living in endemic areas of multiple infectious diseases such as dengue virus, Zika virus and chikungunya virus, it is necessary to carry out rapid detection of multiple pathogens simultaneously to prevent missed diagnosis or misdiagnosis.