Abstract: Objective To discuss comprehensive intervention effect on the control of hospital infections in the intensive care unit(ICU) of a hospital by monitoring Staphylococcus aureus infections and their drug resistance.Methods Comparative analysis was done retrospectively in separation results of Staphylococcus aureus between 2011 and 2012 in ICU patients of a hospital.Results Between 2011 and 2012, there was no obvious difference found in relevance ratio of Staphylococcus aureus(P>0.05), but that of methicillin resistant Staphylococcus aureus was on the decline significantly(P<0.05). The drug resistance rates of Staphylococcus aureus to oxacillin, ciprofloxacin, levofloxacin were on the decline significantly(P<0.05). The drug susceptibility rates of Staphylococcus aureus to vancomycin, teicoplanin, linezolid, nitrofurantoin and primaquine slave tianeptine/dafoe tianeptine were the highest, reaching up to 100.00%.Conclusion By comprehensive intervention, Staphylococcus aureus infections in ICU have been improved and drug resistance rates on the decline as a whole.