Abstract: Objective To discuss the microbiology and epidemiology in food-borne diarrhea cases in a 3-A hospital in Pudong New Area, so as to provide evidence for prevention measures. Methods The data on food-borne diarrhea cases was collected from August 14, 2013 to August 13, 2014 in a 3-A general hospital in Pudong New Area and its prevalent features were analyzed with descriptive epidemiologic methods. Stool samples were collected to detect pathogenic bacteria. Results Of the 310 food-borne diarrhea cases, the main suspected food categories were meat and its products(n=115, 37.1%), vegetables and fruits(n=86, 27.7%) and seafood(n=68,21.9%). In 182 stool samples collected, 32 pathogenic strains were detected. The main bacteria was The diarrheagenic Escherichia coli (n=16, 8.80%), Salmonella (n=8,4.40%), and Vibrio parahaemolyticus (n=8, 4.40%). Conclusion Food-borne diarrhea cases found in this 3-A hospital were mainly caused by bacteria as the diarrheagenic Escherichia coli, Salmonellas and Vibrio parahaemolyticus. And meat and its products, vegetables and fruits were possible contaminated food.