ObjectiveTo ascertain the usage of Toxoplasma Gondii(T. gondii) test kit, and analyze the trends of T. gondii infection in Shanghai pregnant women so as to provide scientific evidence for effective intervention measures to prevent congenital toxoplasmosis.

MethodsThe data were collected on methods and results of screening T. gondii infection in pregnant women in Shanghai from 2003 to 2010, and analysis of T. gondii infection was made on pregnant women in this city.

ResultsDuring 2003 and 2010, in 30 hospitals of the City was carried out the screening test for pregnant women T. gontii infection. The overall serological positive rate to T. gondii was 1.46% from the 30 hospitals. The positive rates of IgG antibody was 2.46%(1.48%-4.96%) in 277 523 persons, that of IgM antibody was 0.83%(0.38%-1.48%) in 439 868 persons. The IgM or both IgG and IgM positive pregnancy cases accounted for 41.74% of the total positive cases which appeared in pregnant women from 2008 to 2010. There were also significant differences in the positive rates in different hospitals and so as in different seasons. ELISA and MEIA were more detected for T. gondii antibody detection, and their detection rates of IgG and IgM were significantly different. The imported kit has higher positive rate of detection T. gondii IgM antibody, but lower positive rate of screening IgG antibody than the domestic kit.

ConclusionThe seroprevalence of T. gondii in pregnant women from 2003 to 2010 were close to the average level investigated in the past in Shanghai, but more attention should be paid to the higher proportion of IgM antibody positive, and stable and reliable test kits should be selected for detecting T. gondii IgG and IgM antibodies to avoid leak and mistaken diagnosis.