ObjectiveTo ascetain the pathogen spectrum of acute hemorrhagic conjunctivitis and the epidemiologic characteristics in Changning District of Shanghai.

MethodsConjunctival swabs were collected from outpatients with acute conjunctivitis from May 2014 to May 2015 at surveillance hospital in Shanghai Changning District. All Specimens were tested for universal enterovirus, enterovirus subtype 70, adenovirus and coxsackievirus A24, coxsackievirus A24 variant by real-time PCR or RT-PCR. PCR reactions were performed for amplification of partial adenovirus hexon genes. The evolution of amplified genes of selected adenovirus was analyzed by Lasergene and MEGA softwares.

ResultsOf 144 samples of acute conjunctivitis, 22.22% (32/144) were universal enterovirus and adenovirus positive, without EV70 and CA24v detected. Adenovirus positiveness were found in 28 samples, accounting for 87.50% (28/32) of all positive samples. The differences in the positive rates of adenovirus were statistically significant between different age groups, with the highest positive rate in the groups of age 20-39. After sequence analysis was done on all positive samples of adenovirus, it was proved that sequencing achieved success in 5 samples. The sequence information was shown to be adenovirus group D; nucleic acid sequence homology reached 97.6%.

ConclusionThe main pathogens of acute hemorrhagic conjunctivitis is adenovirus group D in 2014-2015 in Changning District of Shanghai, which should be given attention in future monitoring.