上海市松江区急性呼吸道感染儿童肠道病毒感染状况

陆利君; 杨丽华; 乔雪飞; 俞家莉; 崔晓青; 滕峥; 张曦

doi:10.19428/j.cnki.sjpm.2017.09.011

上海市松江区急性呼吸道感染儿童肠道病毒感染状况

Entrovirus infection status in children with acute respiratory infection in Songjiang District of Shanghai

摘要

摘要:
目的了解上海市松江区急性呼吸道感染(ARTI)儿童中肠道病毒(EV)感染情况。
方法收集2015年1—12月因ARTI在松江区中心医院就诊患儿的鼻咽拭子标本1 048份，应用荧光Real Time-PCR法检测标本中EV感染状况，应用RD细胞对EV检测阳性的标本进行病毒分离。结果1 048份标本中检出228例EV阳性, 阳性检出率为21.76%，对EV阳性标本进行病毒分离，共分离到145例EV病毒毒株, 分离率为63.60%(145/228)。5—6月共检出EV病毒83份，占全年EV检出总数的7.92%(83/1 048)，选择病毒分离株中荧光Real Time-PCR检测Ct值较高的40株进行测序比对，其中27株为CoxB₃型, 7株为CoxA₄, 3株为CoxA₆, 3株为CoxA₁₀。6月龄~6岁年龄段的儿童EV检出率最高。
结论2015年上海市松江区ARTI儿童中EV感染占21.76%，主要以EV-B型为主。应用分子分型技术对儿童ARTI中的EV病原体进行监测研究，可为相关疾病的流行或暴发提供实验室依据，也可为本地区儿童ARTI的诊治提供实验依据。

Abstract:
ObjectiveTo ascertain the infection status of entrovirus(EV) in children with influenza-like-illness in Songjiang District of Shanghai.
MethodsA total of 1 048 nasopharyngeal swab specimens were collected from children with acute respiratory tract infection (ARTI) during Jan 2015-Dec 2015. Samples were detected by Real-time PCR for EV infection status. With EV test positive specimens, entrovirus strains were isolated from general cell culture methods with RD cells.
ResultsOf the 1 048 swab specimens, 228 were detected to be EV positive. The positive detection rate was 21.76%. And 145 cases of EV strains were isolated from these positive samples. The isolation rate was 63.60% (145/228). Eighty three entroviruses were detected during May 2015-June 2015. The detection rate was 7.92%(83/1 048) of the total in whole year. Forty entrovirus strains were chosen with high Ct values to compare by sequence. The result showed that 27 strains were CoxB₃, 7 strains CoxA₄, 3 strains CoxA₆, and 3 strains CoxA₁₀. In patients aged 6 months-6 years, viral detection rates were higher than those in other groups.
ConclusionType-B EV is the major entrovirus in children with acute respiratory infection in Songjiang District of Shanghai in 2015, whose detection rate was 21.76% in whole year. Therefore, it is important to develop and apply molecular typing method to monitor the entrovirus in children with acute respiratory infection, which offers the information of laboratory for disease prevention and diagnosis and also the laboratory reason why there is the outbreak or epedamic of the disease.

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