积分波微磁场对PM2.5气管滴注致大鼠血液中炎症因子及氧化应激指标变化的影响
Effects of pulsed electromagnetic field on PM2.5 -induced inflammatory and oxidative stress changes in rat's serum
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摘要:目的观察积分波微磁场对大气PM2.5混悬液气管滴注引起的大鼠血液中炎症及氧化应激变化的影响。方法健康成年雄性SD大鼠以每组6只随机分为6组。空白组不做任何处理; 生理盐水对照组仅气管滴注生理盐水; PM2.5暴露组仅气管滴注PM2.5混悬液40 mg/kg; 积分波微磁场干预组先进行PM2.5混悬液气管滴注, 后积分波微磁场治疗仪分别以每天3次、5次的频率进行干预; 积分波微磁场干预对照组仅以每天5次的频率进行处理。末次处理24 h后, 收集大鼠血清, 测定血清中的肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)。结果与生理盐水对照组相比, 滴注了PM2.5混悬液的各组大鼠血清中TNF-α、IL-1β及MDA水平增高, GSH-Px活力下降, 除积分波微磁场(每天3次)干预组外, 其余滴注了PM2.5混悬液的各组T-SOD活力下降。与PM2.5暴露组相比, 积分波微磁场(每天3次)干预组血清中TNF-α、IL-1β水平降低, GSH-Px及T-SOD活力升高, 差异均有统计学意义。结论积分波微磁场治疗对PM2.5混悬液气管滴注引起的大鼠血液中炎症及氧化应激变化有一定的干预作用。Abstract:Objective To evaluate the effects of pulsed electromagnetic field (PEMF) therapy on inflammatory and oxidative stress damage caused by ambient fine particulate matter (PM2.5) in rat's serum.MethodsThirty-six male Sprague-Dawley rats were randomly allocated in six groups (n=6 each): Blank control group: the rats were given nothing to treat. Saline control group: the rats were exposed to normal saline by instillation. PM2.5 control group: rats were exposed to PM2.5 saline suspension by intra-tracheal instillation every other day for three times with the dose of 40mg/kg body weight. PEMF treatment groups: two groups of rats were treated with active PEMF for 3 or 5 cycles per day after exposed to PM2.5 saline suspension. PEMF control group: rats were treated with active PEMF for 5 cycles per day without PM2.5 saline suspension exposure. Saline and PM2.5 were given in the first five days. From the sixth day to nineteenth day, active PEMF therapy was given. Rat's serum was collected 24 hours after the last treatment cycle to analyze the markers related to inflammation and oxidative stress.ResultsThe tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and malondialdehyde (MDA) in rat's serum of PM2.5-exposed groups were significantly higher than those of saline control group. Meanwhile, glutathione peroxidase (GSH-Px) activity was decreased in PM2.5-exposed groups. Compared with PM2.5 control group, the contents of TNF-α, IL-1β and MDA were decreased and the activities of GSH-Px and total-superoxide dismutase (T-SOD) were increased in PEMF treatment group (3 cycles per day). There were no significant differences among the groups of blank control group, saline control group and PEMF control group.ConclusionsPEMF treatment may show antagonistic effects on inflammatory and oxidative stress damage caused by PM2.5 in rat's serum.
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