清肺抑火中药干预大气PM<sub>2.5</sub>对大鼠肺部炎症损伤的实验研究

杨莹莹; 刘洁; 蒋蓉芳; 张静; 宋伟民

doi:10.19428/j.cnki.sjpm.2017.11.007

清肺抑火中药干预大气PM_2.5对大鼠肺部炎症损伤的实验研究

Traditional Chinese medicine intervention effect on rats' pulmonary inflammation damage induced by PM_2.5

摘要

摘要:
目的探讨中药清肺抑火中药对PM_2.5致大鼠肺部炎症损伤的干预作用。
方法将42只健康成年Wistar雄性大鼠随机分为7组，分别为空白对照组、生理盐水对照组、干预对照组、染毒对照组、低剂量干预组、中剂量干预组和高剂量干预组。处理结束后，收集各组大鼠肺泡灌洗液(BALF)，并测定BALF中生化指标和炎症因子水平的变化。
结果与生理盐水对照组比较，染毒对照组BALF中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)的活力和总蛋白(TP)、白蛋白(ALB)、白介素-1β(IL-1 β)、白介素-6(IL-6)、肿瘤坏死因子(TNF-α)、超敏C反应蛋白(HS-CRP)含量均升高，差异有统计学意义(P < 0.05)。低、中、高剂量干预组BALF中生化指标和炎症因子水平比染毒对照组水平降低，差异有统计学意义(P < 0.05)。
结论清肺抑火中药可能通过抗细胞毒性和抗炎症作用，减轻PM_2.5暴露对大鼠肺部产生的损伤。

Abstract:
ObjectiveTo explore the intervention effect of Qingfeiyihuo capsule on rats' pulmonary inflammation damage induced by fine particulate matter (PM_2.5).
MethodsForty-two healthy adult male Wistar rats were randomly divided into seven groups:blank group: saline group, intervening control group, PM_2.5-exposure group, and intervening groups (low, moderate and high-dose).The levels of ACP, AKP, LDH, TP, ALB, IL-1β, IL-6, TNF-α and HS-CRP in brouchoalveolar lavage fluid (BALF) were analyzed.
ResultsThe levels of ACP, AKP, LDH, TP, ALB, IL-1β, IL-6, TNF-α and HS-CRP in the PM_2.5-exposure group were higher than those in the saline group, whose differences were statistically significant(P < 0.05).The levels of ACP, AKP, LDH, TP, ALB, IL-1β, IL-6, TNF-α and HS-CRP in the intervening groups were lower than those in the PM_2.5-exposure group with statistical significances (P < 0.05).
ConclusionsQingfeiyihuo capsule may reduce the pulmonary inflammation damage induced by PM_2.5.

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清肺抑火中药干预大气PM2.5对大鼠肺部炎症损伤的实验研究

Traditional Chinese medicine intervention effect on rats' pulmonary inflammation damage induced by PM2.5

清肺抑火中药干预大气PM_2.5对大鼠肺部炎症损伤的实验研究

Traditional Chinese medicine intervention effect on rats' pulmonary inflammation damage induced by PM_2.5