Veriflow DNA签名捕获技术在检测食品中单增李斯特菌的应用

王颖; 王皓; 董坚

doi:10.19428/j.cnki.sjpm.2018.18824

Veriflow DNA签名捕获技术在检测食品中单增李斯特菌的应用

Application of Veriflow on detection of Listeria monocytogenes in food

摘要

摘要:
目的建立快速检测食品中单增李斯特菌的实验方法。
方法与国家标准方法对比研究Veriflow方法检测单增李斯特菌的效能，对3种食品样品和4种环境表面样品进行单增李斯特菌的定性检测，以建立和验证快速检测食品中单增李斯特菌的实验方法。
结果Veriflow方法的检测结果与国标方法的结果差异无统计学意义(P＞0.05)。Veriflow方法的特异性验证结果显示该法对单增李斯特菌检测的包容性和特异性均为100%。Veriflow方法的检测时间只需26 h，短于国家标准的检测时间(6 d)，检测效率明显提高。
结论Veriflow是一种准确、特异性强并且操作简单的单增李斯特菌快速检测方法，具有良好的推广前景。

Abstract:
ObjectiveTo explore the application of fast detection of Listeria monocytogenes in food.
MethodsAfter comparison the performance of Veriflow method with Chinese National Standard (GB) method in detecting Listeria monocytogenes, the 7 samples, 3 from food productions and 4 from surface of food packages were analyzed.
ResultsThere is no significant difference between the Veriflow and GB method.Among specificity test, the Veriflow got 100 percent in terms of inclusivity and exclusivity.This method had a testing period of 26 hours, less than the GB method′s 6 days, which means a more effective improvement so then.
ConclusionVeriflow is a sensitive, selective, and fast method for the detection of L.monocytogenes sampled from environmental and food matrixes, with a bright future for further application accordingly.

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