陈雪芳, 许文芳. 慢性乙型肝炎患者外周血CD4+和CD8+T细胞中微小RNA-155水平[J]. 上海预防医学, 2019, 31(3): 238-241. DOI: 10.19428/j.cnki.sjpm.2019.18583
引用本文: 陈雪芳, 许文芳. 慢性乙型肝炎患者外周血CD4+和CD8+T细胞中微小RNA-155水平[J]. 上海预防医学, 2019, 31(3): 238-241. DOI: 10.19428/j.cnki.sjpm.2019.18583
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CHEN Xue-fang, XU Wen-fang. MicroRNA-155 levels in peripheral blood CD4+ and CD8+ T cells of chronic hepatitis B patients[J]. Shanghai Journal of Preventive Medicine, 2019, 31(3): 238-241. DOI: 10.19428/j.cnki.sjpm.2019.18583
Citation: CHEN Xue-fang, XU Wen-fang. MicroRNA-155 levels in peripheral blood CD4+ and CD8+ T cells of chronic hepatitis B patients[J]. Shanghai Journal of Preventive Medicine, 2019, 31(3): 238-241. DOI: 10.19428/j.cnki.sjpm.2019.18583
shu

慢性乙型肝炎患者外周血CD4+和CD8+T细胞中微小RNA-155水平

MicroRNA-155 levels in peripheral blood CD4+ and CD8+ T cells of chronic hepatitis B patients

    摘要
  • 摘要:
    目的研究慢性乙型肝炎(慢乙肝)患者外周血微小RNA(miR)-155水平及其与免疫活化的关系。
    方法收集81例慢乙肝患者、77例HBV携带者和51例正常对照者。检测肝功能、HBV DNA和乙肝三系，用流式细胞术检测外周血CD4+和CD8+T细胞水平及其活化水平(CD38+比例)。免疫磁珠分选外周血CD4+和CD8+T细胞，提取总RNA，实时定量PCR法检测CD4+和CD8+T细胞中相对miR-155水平。
    结果慢乙肝组外周血CD4+和CD8+T细胞中相对miR-155水平分别为689.37±139.43和2735.05±397.56，高于HBV携带组(420.64±101.32和1287.46±323.51)和对照组(271.83±72.95和624.62±158.33)的水平(P＜0.05)，HBV携带组CD8+T细胞中相对miR-155水平高于对照组(P＜0.01)。慢乙肝组CD4+和CD8+T细胞活化水平分别为(52.93±8.64)%和(69.28±14.85)%，高于对照组(22.34±5.98)%和(28.64±9.53)%(P＜0.01)，并且与miR-155水平呈正相关。
    结论慢乙肝患者外周血CD4+和CD8+T细胞中相对miR-155水平显著升高，可能与免疫活化呈正相关。

     

    Abstract:
    ObjectiveTo study the miR-155 levels in peripheral blood of patients with chronic hepatitis B and its relationship with immune activation.
    MethodsEighty-one patients with chronic hepatitis B (CHB), 77 HBV carriers and 51 healthy controls were recruited.HBV DNA and serologic tests were carried out for each subject.Levels of miR-155 in peripheral blood were detected by quantitative reverse transcription-polymerase chain reaction.Immune activation of T cells was determined by detection of surface molecules CD38 using flow cytometry.
    ResultsWe found higher miR-155 levels in CD4+ and CD8+ T cells of CHB patients (689.37±139.43 and 2 735.05±397.56) than in HBV carriers (420.64±101.32 and 1 287.46±323.51) or healthy controls (271.83±72.95 and 624.62±158.33) (P < 0.01), and miR-155 levels in CD8+ T cells of HBV carriers were higher than in healthy controls(P < 0.01). Furthermore, immune activation of CD4+ and CD8+ T cells (52.93± 8.64)% and(69.28±14.85)% in CHB patients was much higher than that in healthy controls (22.34± 5.98)% and (28.64±9.53)% (P < 0.01).
    ConclusionmiR-155 expression in CD4+ and CD8+ T cells of CHB patients is increased and positively correlated with T cell activation.

     

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