Abstract: During the 2010 Shanghai Expo, the government regulatory and relevant departments made an all-out effort to guarantee food safety.Faced with severe challenges such as long duration, high tourist flow, high degree of openness, low self-sufficiency and complex food supplying methods, the government departments adopted new concepts, new methods and tools of public health used in recent years to implement full-scale supervision, source control, prevention first, regional classifying and responsibility sharing to effectively solve series of problems and obtained successful experience in safeguarding major national activities.This paper retrospectively reviewed the organizational structure, development of safeguards, technical support, strict on-site supervision, innovation and safeguard measures, risk communication, and implementation of linkage prevention in relation to food safety guarantee for Shanghai Expo.