Abstract:
ObjectiveTo analyze the first suspected dengue fever case in Dapeng New District by characterizing the virus and exploring its origin.
MethodsDengue virus nucleic acid in blood sample was identified by real-time fluorescent RT-PCR. The E gene of dengue virus was amplified and sequenced. Homology and phylogenetic tree of this dengue virus were compared with the isolates from other regions.
ResultsNucleic acid of dengue virus type 1 was detected in the serum sample from the suspected dengue fever patient. Phylogenetic tree showed that the homology based on nucleotide sequence of E gene of DP-DGR19001 was very similar with the isolates of MG894965/TW/CHN/2016 (99.87%), LC428079/VNM/2017(99.80%), MG894867/TW/CHN/2015 (99.60%) and MN512242/GX/CHN/2014 (99.46%). The deduced amino acid sequence of 5 isolates was 100% identical. The patient had traveled to high-incidence country Cambodia in 14 days before infection.
ConclusionsThe first suspected dengue fever case in Dapeng New District is caused by dengue virus type 1. The case is an imported dengue fever .