高效液相色谱法测定化妆品中L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的含量

许勇; 韩晶; 潘晨; 彭兴盛; 郑荣

doi:10.19428/j.cnki.sjpm.2021.20083

高效液相色谱法测定化妆品中L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的含量

A method for examination of L-carnosine， eyeseryl，glutathione and acetyl hexapeptide-8 in cosmetics by HPLC

摘要

摘要:
目的建立高效液相色谱法测定化妆品中L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的测定方法。
方法样品经水提取，以Agilent Eclipse XDB-C18 （5 μm，4.6 mm×150 mm）液相色谱柱进行分离，以甲醇和含有0.1%甲酸（体积分数，V/V）水溶液梯度洗脱，流速0.5 mL/min，检测波长210 nm，经外标法定量。
结果L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的线性范围均为5～100 μg/mL，r值均为0.999 9；检出限均为30 mg/kg，L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8加标回收率（n＝6）范围为92.5%～105.9%，精密度（相对标准偏差，RSD）为0.5%～3.5%。
结论该方法灵敏度高、专属性好、操作简便、重现性好，可用于化妆品中L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的测定。

Abstract: ［
ObjectiveTo establish a HPLC method for examination of L-carnosine，eyeseryl，glutathione and acetyl hexapeptide-8 in cosmetics.
MethodAfter being extracted by water， L-carnosine， Eyeseryl， Glutathione， and Acetyl Hexapeptide-8 were examined by HPLC with methanol-0.01% formic acid （V/V） aqueous solution as the mobile phase. The column was Agilent Eclipse XDB-C18 （5 μm， 4.6 mm×150 mm）. The flow rate was 0.5 mL/min. The determination wavelength was set at 210 nm.
ResultsThere was a good linear relationship within the range of 5-100 μg/mL for L-carnosine， eyeseryl， glutathione， and acetyl hexapeptide-8. The recoveries of L-carnosine Eyeseryl Glutathione and Acetyl Hexapeptide-8 were between 92.5%~105.9%， with a RSD from 0.5% to 3.5%.
ConclusionThe method is simple， sensitive， specific and reproducible in the examination of L-carnosine， Eyeseryl， Glutathione， and Acetyl Hexapeptide-8 in cosmetics.

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