杨博雯, 叶玉龙, 袁佳春, 阙凤霞, 陈洪友. 上海市金山区腹泻患者中分离的副溶血弧菌病原学特征研究[J]. 上海预防医学, 2021, 33(8): 714-719. DOI: 10.19428/j.cnki.sjpm.2021.20380
引用本文: 杨博雯, 叶玉龙, 袁佳春, 阙凤霞, 陈洪友. 上海市金山区腹泻患者中分离的副溶血弧菌病原学特征研究[J]. 上海预防医学, 2021, 33(8): 714-719. DOI: 10.19428/j.cnki.sjpm.2021.20380
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YANG Bo-wen, YE Yu-long, YUAN Jia-chun, QUE Feng-xia, CHEN Hong-you. Characterization of Vibrio parahaemolyticus isolated from diarrhea patients in Jinshan District，Shanghai[J]. Shanghai Journal of Preventive Medicine, 2021, 33(8): 714-719. DOI: 10.19428/j.cnki.sjpm.2021.20380
Citation: YANG Bo-wen, YE Yu-long, YUAN Jia-chun, QUE Feng-xia, CHEN Hong-you. Characterization of Vibrio parahaemolyticus isolated from diarrhea patients in Jinshan District，Shanghai[J]. Shanghai Journal of Preventive Medicine, 2021, 33(8): 714-719. DOI: 10.19428/j.cnki.sjpm.2021.20380
shu

上海市金山区腹泻患者中分离的副溶血弧菌病原学特征研究

Characterization of Vibrio parahaemolyticus isolated from diarrhea patients in Jinshan District，Shanghai

    摘要
  • 摘要:
    目的了解2016—2018年上海市金山区副溶血弧菌的分布和流行特征。
    方法对218株分离自腹泻患者的副溶血弧菌进行血清型分析及不耐热性溶血素（TLH）、耐热性溶血素（TDH）、TDH类似溶血毒（TRH）毒力基因测定和药敏试验并进行脉冲场凝胶电泳（PFGE）分子分型。
    结果218株副溶血弧菌可分为8个血清群，21个血清型，其中147株可获得明确血清分型结果，以O3：K6最多，达57.3%；所有受试菌株按照85%相似度水平可分为25个簇类，优势簇为JSVP02，总相似度为56.3%~100.0%；201株菌携带tdh基因，阳性率为92.2%，13株携带trh基因，阳性率为6.0%，此外7株菌tdh、trh检测结果均为阴性；35株菌对所用17种抗菌药物完全敏感，其余183株菌呈现不同程度耐药。
    结论2016—2018年金山区腹泻患者中分离的副溶血弧菌遗传分布多样化，大部分菌株具有tdh基因，对第一代头孢菌素类的头孢唑啉、青霉素类的氨苄西林耐药较严重。通过构建区域化的PFGE分子分型数据库将对高危菌株的筛查、识别、预警提供科学依据。

     

    Abstract:
    ObjectiveTo determine the distribution and epidemic characteristics of Vibrio parahaemolyticus in Jinshan District of Shanghai from 2016 through 2018.
    MethodsSerotype analysis，examination of virulence genes， including thermolabilehemolysin（TLH），thermostable direct hemolysin（TDH），and TDH related hemolysin（TRH），and antimicrobial susceptibility test and pulsed field gel electrophoresis （PFGE） molecular typing were performed on 218 Vibrio parahaemolyticus isolated from diarrhea patients.
    ResultsA total of 218 strains were divided into 8 groups and 21 serotypes. Of them， 147 strains were serotyped，with O3：K6 as the most common serotype （57.3%）. All tested strains were divided into 25 clusters based on the similarity of 85% and above，in which the dominant cluster was JSVP02，and the total similarity was 56.3%-100.0%. Two hundred and one strains （92.2%） carried tdh gene，13 strains （6.0%） carried trh gene，and 7 strains were negative for both tdh and trh. A total of 35 strains were completely sensitive to 17 kinds of antibiotics，while the remaining 183 strains showed different drug resistance.
    ConclusionVibrio parahaemolyticus isolated from diarrhea patients in Jinshan District from 2016 through 2018 is diverse. Majority of the strains have TDH gene and are resistant to the first generation cephalosporins such as cefazolin and penicillin ampicillin. Construction of regional PFGE molecular typing database will facilitate screening，identification and early warning of high-risk strains.

     

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