化妆品睫毛增长液中前列腺素类似物的高效液相色谱-串联质谱法测定

许勇; 潘晨; 韩晶; 彭兴盛; 郑荣

doi:10.19428/j.cnki.sjpm.2022.20643

化妆品睫毛增长液中前列腺素类似物的高效液相色谱-串联质谱法测定

Determination of five prostaglandin analogs in eyelash enhancing cosmetics by LC-MS/MS

摘要

摘要:
目的建立液相色谱-串联质谱（LC⁃MS/MS）法测定睫毛增长液中比马前列素、他氟乙酰胺、拉坦前列素、曲伏前列素和他氟前列素的分析方法。
方法样品经50%乙腈的水溶液超声提取，加入4 g NaCl和1 g MgSO₄的混合盐沉淀杂质，离心。上层有机相经微孔滤膜过滤后，用Agilent Poroshell 120 PFP⁃C18柱（2.7 μm，2.1 mm×100 mm）分离，以含5 mmol·L^-1乙酸铵的0.02%甲酸-乙腈为流动相，梯度洗脱，流速0.5 mL·min^-1。采用电喷雾正离子源（ESI⁺），多反应监测（MRM）扫描方式检测，外标法定量。
结果比马前列素、他氟乙酰胺、拉坦前列素、曲伏前列素和他氟前列素在试验范围内线性良好，r>0.999。考察了膏霜、乳液、凝胶、水4种常见睫毛增长液基质的 3个水平的加标回收率。结果回收率均在93.2%~103.5%，相对标准偏差（RSD）均在1.2%~3.4%，检出限（LOD）在0.000 2~1.5 μg·g^-1。在测定的63批睫毛增长液中，检出比马前列素15批。
结论该方法快速简便，灵敏度高，适用于市售睫毛增长液化妆品中上述5种前列素类似物的测定。

Abstract:
Objective To determine bimatoprost， tafluprost ethyl amide， latanoprost， travoprost and tafluprost in eyelash enhancing cosmetics by establishing a LC-MS/MS method.
Methods The samples were extracted with a 50% acetonitrile water solution. A salt mixture （4 g NaCl， 1 g MgSO₄） was added to the solution to induce phase separation. After centrifugation and filtration， the analysis of five prostaglandin analogs was performed with an Agilent Poroshell 120 PFP-C18 （2.7 μm， 2.1 mm×100 mm） column， using 0.02% formic acid containing 5 mmol·L^-1 Acetic acid amine and acetonitrile by gradient elution at a flow rate of 0.5 mL·min^-1. The analytes were detected with electrospray ionization source in positive ion mode （ESI⁺） and multiple reaction monitoring （MRM）， and quantified by external standard curve.
Results The results showed that it had a good linearity in the range of locatable ambit of concentration^{with correlation coefficients （r） larger than 0.999. The detection limit of five prostaglandin analogs （LOD） was 0.000 2‒1.5 μg·g^-1. The spiked recoveries were 93.2% to 103.5% with a relative standard deviation （RSD） of 1.2% to 3.4%.}
Conclusion The method is simple， rapid and highly sensitive. It is suitable for the determination of five prostaglandin analogs in eyelash enhancing cosmetics.

HTML全文

参考文献(22)

施引文献

资源附件(0)