陈培超, 黄强, 孙攀, 董玉婷, 彭谦. 20192020年上海市嘉定区市售禽肉中沙门菌耐药性分析[J]. 上海预防医学, 2022, 34(5): 411-416. DOI: 10.19428/j.cnki.sjpm.2022.21510
引用本文: 陈培超, 黄强, 孙攀, 董玉婷, 彭谦. 20192020年上海市嘉定区市售禽肉中沙门菌耐药性分析[J]. 上海预防医学, 2022, 34(5): 411-416. DOI: 10.19428/j.cnki.sjpm.2022.21510
CHEN Peichao, HUANG Qiang, SUN Pan, DONG Yuting, PENG Qian. Analysis of salmonella drug resistance in poultry sold in Jiading District from 2019 to 2020[J]. Shanghai Journal of Preventive Medicine, 2022, 34(5): 411-416. DOI: 10.19428/j.cnki.sjpm.2022.21510
Citation: CHEN Peichao, HUANG Qiang, SUN Pan, DONG Yuting, PENG Qian. Analysis of salmonella drug resistance in poultry sold in Jiading District from 2019 to 2020[J]. Shanghai Journal of Preventive Medicine, 2022, 34(5): 411-416. DOI: 10.19428/j.cnki.sjpm.2022.21510

20192020年上海市嘉定区市售禽肉中沙门菌耐药性分析

Analysis of salmonella drug resistance in poultry sold in Jiading District from 2019 to 2020

  • 摘要:
    目的 了解上海市嘉定区市售禽肉中沙门菌污染状况、血清型分布及其耐药性。
    方法 采集2019—2020年市售的鸡、鸭、鹅和鸽4类生食禽肉样本进行沙门菌分离鉴定,并对分离的沙门菌进行血清型分型和13种常见抗生素耐药性检测。
    结果 156份市售禽肉样品中分离到沙门菌32株。鹅肉中沙门菌污染情况最为严重,检出率为28.00%,鸽肉中未检出沙门菌。冷冻、冷藏和室温存储样品中,冷冻状态下沙门菌检出率为26.67%,冷藏状态检出率最低,为12.90%。血清型以鼠伤寒沙门菌、肠炎沙门菌和阿贡纳沙门菌为主。沙门菌对萘啶酸、四环素的耐药率较高,耐药率分别为59.38%和40.63%;对头孢类药物的耐药率较低;对亚胺培南未表现出耐药性。鸡、鸭、鹅不同类别里检出的沙门菌耐药差异显著,3种主要血清型沙门菌耐药也有所不同。21.88%(7/32)沙门菌为不耐药,多重耐药菌株占比为31.25%(10/32),耐药数最多达11种。
    结论 上海市嘉定区市售4类禽肉样本沙门菌的污染状况及耐药性有差异,应进行分类监督管理。

     

    Abstract:
    Objective To investigate the contamination situation, serotype distribution and drug resistance of salmonella in poultry sold in Jiading District, Shanghai.
    Methods Between 2019 and 2020, four types of poultry meat (chicken, duck, goose and pigeon) were sampled from markets, and potential salmonella contamination was isolated and identified via serotyping. Furthermore, resistance detection of isolated salmonella to 13 commonly-used antibiotics was conducted.
    Results A total of 32 salmonella strains were isolated from 156 commercial poultry samples. No salmonella was identified in pigeon samples, while the most severe contamination was detected in goose samples, with a detection rate of 28.00%. Contamination situation was categorized with different storage conditions. The lowest detection rate of 12.90% was noted under cold storage, while the highest rate of 26.67% was detected under frozen condition. The major serotypes of salmonella were Salmonella typhimuriumSalmonella enteritidis and Salmonella Argonne. Relatively higher drug resistance was detected with nalidixic acid and tetracycline, with drug resistance rate of 59.38% and 40.63%, respectively. Low drug resistance was detected with cephalosporins, and no drug resistance was detected with imipenem. Significant difference in drug resistance was noted in the serotype of Salmonella isolated from meat of chicken, duck and goose. Three major salmonella strains had different drug resistance. 21.88% (7/32) of salmonella strains were non-resistant to any tested drugs. Multi-drug resistant strains accounted for 31.25% (10/32) of the isolated strains. At most, salmonella was resistant to 11 kinds of different antibiotics.
    Conclusion There is a significant difference in the contamination situation and drug resistance of Salmonella isolated from four types of poultry meat sold in Jiading District, Shanghai. It is strongly recommended that different supervision strategies and management upon classification be implemented.

     

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