Abstract: Objective To analyze the implementation effect of the Global Fund for tuberculosis (TB) control project on designated hospital, and provide basis and recommendation for TB control in Wenzhou City of Zhejiang Province. Methods According to the data from the National TB Management Information System,quarterly reports of project were collected and analyzed. Results The X-ray check rate and sputum test rate of newly diagnosed patients both reached more than 97%. The registration rate of active pulmonary TB and smear-positive TB were 71.45 per 100 000 and 26.41 per 100 000. A total of 1 393 new smear-positive pulmonary TB cases were detected. The completion of case finding target for new smear-positive pulmonary TB was 119.67%. The cure rate and treatment success rate for new smear-positive cases were 88.08% and 90.52%. And 1 657 cases have accepted psychological support. The psychological support rates were 36.05%. A total of 39 361 person times for TB cases received free liver function test, and the test rate was 100%. Conclusion This project was implemented well in Wenzhou, and obtained good achievement. The discovery of new smear-positive patients, treatment outcome, psychological support and free liver function test for TB patients have reached the project requirements.