Abstract: Objective To analyze the multidrug resistant situation with Mycobacterium tuberculosis in Kaihua county in order to provide technical basis for formulating measures on tuberculosis control. Methods Mycobacterium tuberculosis were cultivated, which were collected from smear positive tuberculosis patients' sputum in People's Hospital of Kaihua County from January 2010 to December 2013. Proportion method for Mycobacterium tuberculosis drug sensitivity test was conducted to 6 anti-tuberculosis drugs (isoniazide, rifampin, ethambutol, streptomycin, levofloxacin, kanamycin). Results In 219 Mycobacterium tuberculosis culture positive patients, the rate of total drug resistance was 21.46% and the rate of total multidrug resistance 3.20%;the rate of initial drug resistance was 19.69%,and the rate of initial multidrug resistance 0.52%;the rate of acquired drug resistance was 34.62%,and the rate of acquired multidrug resistance 23.08%. Conclusion The rate of total drug resistance, total multidrug resistance, initial drug resistance, initial multidrug resistance and acquired drug resistance are respectively less than the result of The 5^th National Tuberculosis Epidemiological Survey, while the rate of acquired multidrug resistance is more than the latter.