Abstract: Objective To investigate the discrimination status among AIDS medical staff towards HIV/AIDS patients in XuHui district and analyze the factors influencing the discriminary attitude and behavior will of medical staff. Methods A total of 133 AIDS medical staff from 31 medical institutions were investigated by means of a self-designed questionnaire in Xuhui District. Results In the attitude to PLWHA, the terms in the questionnaire with the highest and the lowest discrimination score were "people living with HIV should be sent to infection hospital" and "the privacy of people living with HIV should be respected", whose scores were 1.59 and 0.03 points. In the behavior will to PLWHA, the terms with the highest and the lowest discrimination score were "not to shop from people living with HIV" and "willing to communicate with acquaintances infected with HIV", whose scores were 1.05 and 0.48 points. The difference in discrimination score was female > male and clinical post > test post > healthcare post. Conclusion Gender and occupation are the main factors affecting discrimination scores. The attitude and behavior will to PLWHA are inconsistent in some places. Measures should be taken to reduce PLWHA discrimination, such as effectively implementing the "AIDS prevention and control regulation", strengthening the occupation moral training of medical staff, and enhancing the occupation protection policy and training.