Abstract: Objective To evaluate the effectiveness of AIDS health education for pregnant women in their knowledge,attitude and behavior on AIDS prevention and mother-to-child transmission, so as to provide scientific basis for health education. Methods From 5 suburban hospitals, a total of 800 pregnant women investigated accepted AIDS health education intervention study. Interventions included face to face counseling with medical personnel, distribution of publicity materials of basic AIDS prevention, and preventing mother-to-child transmission of AIDS. Through investigation by two questionnaires done before and after intervention, we obtained the changes in pregnant women's knowledge,attitude and behavior on AIDS prevention and mother-to-child transmission, and thus evaluated the efficacy of the intervention. Results Before health education intervention, pregnant women were found to be in lack of knowledge, and had incorrect attitude and behavior on basic AIDS prevention, and mother-to-child transmission of AIDS prevention. After intervention, the situation markedly improved. Conclusion AIDS health education intervention increases pregnant women's knowledge on basic AIDS prevention, and mother-to-child transmission of AIDS prevention, changes their wrong attitude and behavior, improves self protection consciousness, and reduces their discrimination against AIDS patients. It is clear that we must continue to carry out health education intervention, so as to control the spread of AIDS effectively.