Abstract: Objective To investigate the disease burden level and trend of leukemia in Ningbo City of Zhejiang Province. Methods Disability adjusted life years (DALY) of leukemia was calculated for residents on the basis of leukemia monitoring data in Ningbo City. Results The disease burden of leukemia in Ningbo City was 30 681.26 DALYs; the intensity was 1.06 DALYs per 1 000 population. The disease burden and intensity of leukemia in male was higher than that in female. The disease burden of leukemia was focused on those aged 40 years and above. The disease burden intensity was rising year by year. Conclusion The disease burden of leukemia in Ningbo City has been relatively stable. It is suggested that the health administrative departments should enhance prevention and control of leukemia in those under 10 years and those aged 50 and over, so as to reduce the disease burden resulting from leukemia.